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Int. J. Mol. Sci. 2011, 12(6), 3941-3949; doi:10.3390/ijms12063941
Article

Extracts and Constituents of Rubus chingii with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Free Radical Scavenging Activity

Received: 27 April 2011; in revised form: 1 June 2011 / Accepted: 8 June 2011 / Published: 14 June 2011
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity of the fruits of Rubus chingii was studied in vitro. Ethanolic extract, ethyl acetate and n-butanol fractions from dried R. chingii fruits revealed strong DPPH free radical scavenging activity with IC50 values of 17.9, 3.4 and 4.0 µg/mL, respectively. The ethyl acetate and n-butanol fractions were further purified by a combination of silica gel chromatography, Lobar RP-8 chromatography, and high-pressure liquid chromatography (HPLC). Nine compounds were isolated, where methyl (3-hydroxy-2-oxo-2,3-dihydroindol-3-yl)-acetate (2), vanillic acid (5), kaempferol (7), and tiliroside (9) showed stronger DPPH free radical scavenging activity than that of ascorbic acid (131.8 µM) with IC50 values of 45.2, 34.9, 78.5, and 13.7 µM, respectively. In addition, rubusine (1) is a new compound discovered in the present study and methyl (3-hydroxy-2-oxo-2,3-dihydroindol-3-yl)-acetate (2), methyl dioxindole-3-acetate (3), and 2-oxo-1,2-dihydroquinoline-4-carboxylic acid (4) were isolated from the fruits for the first time.
Keywords: antioxidant; Rubus chingii; 1,1-diphenyl-2-picrylhydrazyl (DPPH); rubusine antioxidant; Rubus chingii; 1,1-diphenyl-2-picrylhydrazyl (DPPH); rubusine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ding, H.-Y. Extracts and Constituents of Rubus chingii with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Free Radical Scavenging Activity. Int. J. Mol. Sci. 2011, 12, 3941-3949.

AMA Style

Ding H-Y. Extracts and Constituents of Rubus chingii with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Free Radical Scavenging Activity. International Journal of Molecular Sciences. 2011; 12(6):3941-3949.

Chicago/Turabian Style

Ding, Hsiou-Yu. 2011. "Extracts and Constituents of Rubus chingii with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Free Radical Scavenging Activity." Int. J. Mol. Sci. 12, no. 6: 3941-3949.



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