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Int. J. Mol. Sci. 2011, 12(5), 3055-3071; doi:10.3390/ijms12053055

Genetic Diversity and Phylogeny of Antagonistic Bacteria against Phytophthora nicotianae Isolated from Tobacco Rhizosphere

1
Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China
2
Zunyi Tobacco Company, Guizhou 564700, China
3
Department of Entomology and Plant Pathology, 209 Life Sciences Bldg, Auburn University, Auburn, AL 36849, USA
4
Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
*
Author to whom correspondence should be addressed.
Received: 12 March 2011 / Revised: 3 April 2011 / Accepted: 20 April 2011 / Published: 12 May 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria.
Keywords: genetic diversity; antagonistic bacteria; Phytophthora nicotianae; tobacco; rhizosphere genetic diversity; antagonistic bacteria; Phytophthora nicotianae; tobacco; rhizosphere
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jin, F.; Ding, Y.; Ding, W.; Reddy, M.; Fernando, W.D.; Du, B. Genetic Diversity and Phylogeny of Antagonistic Bacteria against Phytophthora nicotianae Isolated from Tobacco Rhizosphere. Int. J. Mol. Sci. 2011, 12, 3055-3071.

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