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Int. J. Mol. Sci. 2011, 12(5), 2808-2821; doi:10.3390/ijms12052808

Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben 06466, Germany
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Received: 18 March 2011 / Revised: 11 April 2011 / Accepted: 11 April 2011 / Published: 29 April 2011
(This article belongs to the Special Issue Plant-Derived Pharmaceuticals by Molecular Farming)
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Abstract

Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated. View Full-Text
Keywords: transgenic plants; avian influenza; H5N1; nanobody; ELPylation; Inverse Transition Cycling transgenic plants; avian influenza; H5N1; nanobody; ELPylation; Inverse Transition Cycling
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Phan, H.T.; Conrad, U. Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions. Int. J. Mol. Sci. 2011, 12, 2808-2821.

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