An Oligodeoxynucleotide with Promising Modulation Activity for the Proliferation and Activation of Osteoblast
Abstract
:1. Introduction
2. Results and Discussion
2.1. MTT Assay
2.2. Effect of ODNs on ALP Activity
2.3. Effect of ODN MT01 on the Morphology of MG 63 Cells
2.4. Effect of ODN MT01 on Gene Expression of MG 63 Cells
2.5. Western Blotting Analysis
3. Experimental Section
3.1. Materials
3.2. Cell Culture
3.3. MTT Assay
3.4. Measurement of ALP Activity
3.5. Real-Time PCR
3.6. Western Blotting
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Number | Name | Sequence |
---|---|---|
1 | SAT05f | 5′-CCTCCTCCTCCTCCTCCTCCTCCT-3′ |
2 | MS19 | 5′-AAAGAAAGAAAGAAAGAAAGAAAG-3′ |
3 | BW001 | 5′-TCGTCGGGTGCGACGTCGCAGGGGGG-3′ |
4 | FC001 | 5′-TCGGGGACGATCGTCGGGGAC-3′ |
5 | FC002 | 5′-TCGTCGACGTCGTCGTTCTC-3′ |
6 | BW006 | 5′-TCGACGTTCGTCGTTCGTCGTTC-3′ |
7 | YW002 | 5′-TCGCGAACGTTCGCCGCGTTCGAACGCGG-3′ |
8 | FC004 | 5′-TCGCGTTCGATCGCGATCGACGGTA-3′ |
9 | MT01 | 5′-ACCCCCTCTACCCCCTCTACCCCCTCT-3′ |
10 | YW001 | 5′-TCGCGACGTTCGCCCGACGTTCGGTA-3′ |
11 | FC003 | 5′-TCTCTCTCTCTCTCTCTCTCTCTC-3′ |
Gene Name | Oligonucleotide UP (5′-3′) | Oligonucleotide DW (5′-3′) |
---|---|---|
GAPDH | ATG GGG AAG GTG AAG GTC | TAA AAG CAG CCC TGG TGA CC |
SP7 | CAC AGC TCT TCT GAC TGT CTG CTG | GTG AAA TGC CTG CAT GGA T |
RUNX-2 | GAG ATC ATC GCC GAC CAC | TAC CTC TCC GAG GGC TAC C |
COLLAGEN-I | AGG GCC AAG ACG AAG ACA TC | AGA TCA CGT CAT CGC ACA ACA |
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Feng, Z.; Shen, Y.; Wang, L.; Cheng, L.; Wang, J.; Li, Q.; Shi, W.; Sun, X. An Oligodeoxynucleotide with Promising Modulation Activity for the Proliferation and Activation of Osteoblast. Int. J. Mol. Sci. 2011, 12, 2543-2555. https://doi.org/10.3390/ijms12042543
Feng Z, Shen Y, Wang L, Cheng L, Wang J, Li Q, Shi W, Sun X. An Oligodeoxynucleotide with Promising Modulation Activity for the Proliferation and Activation of Osteoblast. International Journal of Molecular Sciences. 2011; 12(4):2543-2555. https://doi.org/10.3390/ijms12042543
Chicago/Turabian StyleFeng, Zhiyuan, Yuqin Shen, Liying Wang, Lin Cheng, Jing Wang, Quanshun Li, Wei Shi, and Xinhua Sun. 2011. "An Oligodeoxynucleotide with Promising Modulation Activity for the Proliferation and Activation of Osteoblast" International Journal of Molecular Sciences 12, no. 4: 2543-2555. https://doi.org/10.3390/ijms12042543