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Int. J. Mol. Sci. 2011, 12(4), 2518-2542; doi:10.3390/ijms12042518
Review

Functioning Nanomachines Seen in Real-Time in Living Bacteria Using Single-Molecule and Super-Resolution Fluorescence Imaging

1
 and 1,2,*
Received: 28 February 2011; in revised form: 7 April 2011 / Accepted: 11 April 2011 / Published: 15 April 2011
(This article belongs to the Special Issue Molecular Machines and Nanomachines)
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Abstract: Molecular machines are examples of “pre-established” nanotechnology, driving the basic biochemistry of living cells. They encompass an enormous range of function, including fuel generation for chemical processes, transport of molecular components within the cell, cellular mobility, signal transduction and the replication of the genetic code, amongst many others. Much of our understanding of such nanometer length scale machines has come from in vitro studies performed in isolated, artificial conditions. Researchers are now tackling the challenges of studying nanomachines in their native environments. In this review, we outline recent in vivo investigations on nanomachines in model bacterial systems using state-of-the-art genetics technology combined with cutting-edge single-molecule and super-resolution fluorescence microscopy. We conclude that single-molecule and super-resolution fluorescence imaging provide powerful tools for the biochemical, structural and functional characterization of biological nanomachines. The integrative spatial, temporal, and single-molecule data obtained simultaneously from fluorescence imaging open an avenue for systems-level single-molecule cellular biophysics and in vivo biochemistry.
Keywords: fluorescence microscopy; fluorescent protein; in vivo imaging; molecular machine; nanomachine; photobleach; single molecule; slimfield; super-resolution; total internal reflection fluorescence microscopy; fluorescent protein; in vivo imaging; molecular machine; nanomachine; photobleach; single molecule; slimfield; super-resolution; total internal reflection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chiu, S.-W.; Leake, M.C. Functioning Nanomachines Seen in Real-Time in Living Bacteria Using Single-Molecule and Super-Resolution Fluorescence Imaging. Int. J. Mol. Sci. 2011, 12, 2518-2542.

AMA Style

Chiu S-W, Leake MC. Functioning Nanomachines Seen in Real-Time in Living Bacteria Using Single-Molecule and Super-Resolution Fluorescence Imaging. International Journal of Molecular Sciences. 2011; 12(4):2518-2542.

Chicago/Turabian Style

Chiu, Sheng-Wen; Leake, Mark C. 2011. "Functioning Nanomachines Seen in Real-Time in Living Bacteria Using Single-Molecule and Super-Resolution Fluorescence Imaging." Int. J. Mol. Sci. 12, no. 4: 2518-2542.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert