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YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma
Department of Urology, RenMin Hospital of Wuhan University, Wuhan 430060, China
State Key Laboratory of Virology, Wuhan University, Wuhan 430071, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 11 January 2011; in revised form: 2 February 2011 / Accepted: 23 March 2011 / Published: 11 April 2011
Abstract: YueF is a novel putative tumor suppressor gene that can inhibit proliferation and induce apoptosis in hepatoma cells, but its role in renal cell carcinoma (RCC) remains unclear. Here, we examined the expression of the YueF gene in RCC tissues and the effect of YueF on cell proliferation in RCC 786-0 cells. The results showed that YueF was expressed at high levels in normal kidney tissues and cell lines but was reduced or absent in RCC tissues and 786-0 cells. Lentivirus-mediated YueF overexpression in RCC 786-0 cells caused cell-cycle arrest in the G1 phase and dramatically reduced proliferation in culture. YueF overexpression resulted in increased protein levels of p53 and p21WAF1/Cip1, whereas the protein levels of cyclin D1 and pRb were decreased. The proliferation defects caused by YueF overexpression could be partially rescued by the expression of p21 siRNA. These findings suggest a critical role for p21 in the YueF-induced growth inhibition of 786-0 cells and provide novel insights into the mechanism underlying the tumor-suppressive action of YueF.
Keywords: YueF; clear cell RCC; p53; p21
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MDPI and ACS Style
Huang, H.-W.; Peng, J.-P.; Zhang, J. YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma. Int. J. Mol. Sci. 2011, 12, 2477-2487.
Huang H-W, Peng J-P, Zhang J. YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma. International Journal of Molecular Sciences. 2011; 12(4):2477-2487.
Huang, Hsuan-Wei; Peng, Jian-Ping; Zhang, Jie. 2011. "YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma." Int. J. Mol. Sci. 12, no. 4: 2477-2487.