Next Article in Journal
Enhancement of Salinity Tolerance during Rice Seed Germination by Presoaking with Hemoglobin
Previous Article in Journal
Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2011, 12(4), 2477-2487; doi:10.3390/ijms12042477

YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma

1
Department of Urology, RenMin Hospital of Wuhan University, Wuhan 430060, China
2
State Key Laboratory of Virology, Wuhan University, Wuhan 430071, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 11 January 2011 / Revised: 2 February 2011 / Accepted: 23 March 2011 / Published: 11 April 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [378 KB, uploaded 19 June 2014]   |  

Abstract

YueF is a novel putative tumor suppressor gene that can inhibit proliferation and induce apoptosis in hepatoma cells, but its role in renal cell carcinoma (RCC) remains unclear. Here, we examined the expression of the YueF gene in RCC tissues and the effect of YueF on cell proliferation in RCC 786-0 cells. The results showed that YueF was expressed at high levels in normal kidney tissues and cell lines but was reduced or absent in RCC tissues and 786-0 cells. Lentivirus-mediated YueF overexpression in RCC 786-0 cells caused cell-cycle arrest in the G1 phase and dramatically reduced proliferation in culture. YueF overexpression resulted in increased protein levels of p53 and p21WAF1/Cip1, whereas the protein levels of cyclin D1 and pRb were decreased. The proliferation defects caused by YueF overexpression could be partially rescued by the expression of p21 siRNA. These findings suggest a critical role for p21 in the YueF-induced growth inhibition of 786-0 cells and provide novel insights into the mechanism underlying the tumor-suppressive action of YueF.
Keywords: YueF; clear cell RCC; p53; p21 YueF; clear cell RCC; p53; p21
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Huang, H.-W.; Peng, J.-P.; Zhang, J. YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma. Int. J. Mol. Sci. 2011, 12, 2477-2487.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top