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Int. J. Mol. Sci. 2011, 12(4), 2477-2487; doi:10.3390/ijms12042477
Article

YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma

1,†
,
1,†
 and
1,2,*
1 Department of Urology, RenMin Hospital of Wuhan University, Wuhan 430060, China 2 State Key Laboratory of Virology, Wuhan University, Wuhan 430071, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 11 January 2011 / Revised: 2 February 2011 / Accepted: 23 March 2011 / Published: 11 April 2011
(This article belongs to the Section Molecular Pathology)
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Abstract

YueF is a novel putative tumor suppressor gene that can inhibit proliferation and induce apoptosis in hepatoma cells, but its role in renal cell carcinoma (RCC) remains unclear. Here, we examined the expression of the YueF gene in RCC tissues and the effect of YueF on cell proliferation in RCC 786-0 cells. The results showed that YueF was expressed at high levels in normal kidney tissues and cell lines but was reduced or absent in RCC tissues and 786-0 cells. Lentivirus-mediated YueF overexpression in RCC 786-0 cells caused cell-cycle arrest in the G1 phase and dramatically reduced proliferation in culture. YueF overexpression resulted in increased protein levels of p53 and p21WAF1/Cip1, whereas the protein levels of cyclin D1 and pRb were decreased. The proliferation defects caused by YueF overexpression could be partially rescued by the expression of p21 siRNA. These findings suggest a critical role for p21 in the YueF-induced growth inhibition of 786-0 cells and provide novel insights into the mechanism underlying the tumor-suppressive action of YueF.
Keywords: YueF; clear cell RCC; p53; p21 YueF; clear cell RCC; p53; p21
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, H.-W.; Peng, J.-P.; Zhang, J. YueF Overexpression Inhibits Cell Proliferation Partly through p21WAF1/Cip1 Upregulation in Renal Cell Carcinoma. Int. J. Mol. Sci. 2011, 12, 2477-2487.

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