Int. J. Mol. Sci. 2011, 12(4), 2463-2476; doi:10.3390/ijms12042463
Article

Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand

1 Post-Graduate Program in Biological Sciences, Federal University of Pernambuco, CEP 50.670–420, Recife, PE, Brazil 2 Nucleus for Research in Environmental in Environmental Sciences, Catholic University of Pernambuco, Rua Nunes Machado, 42, Bloco J, Térreo, Boa Vista, CEP 50.050–590, Recife, PE, Brazil 3 Post-Graduate Program in Fungal Biology, Federal University of Pernambuco, CEP 50.670–420, Recife, PE, Brazil 4 Center for Sciences and Technology, Catholic University of Pernambuco, Rua do Príncipe, Boa Vista, CEP 50.050–900, Recife, PE, Brazil
* Author to whom correspondence should be addressed.
Received: 1 March 2011; in revised form: 14 March 2011 / Accepted: 23 March 2011 / Published: 8 April 2011
(This article belongs to the Special Issue Biosurfactants)
PDF Full-text Download PDF Full-Text [374 KB, uploaded 8 April 2011 14:46 CEST]
Abstract: Statistical experimental designs and response surface methodology were employed to optimize the concentrations of agroindustrial residues as soybean oil (SORR) from refinery, and corn steep liquor (CSL) from corn industry, for tensio-active agent produced by Candida sphaerica UCP 0995. Three 22 full factorial design were applied sequentially to investigate the effects of the concentrations and interactions of soybean oil refinery residue and corn steep liquor on the surface tension of free-cell culture broth for 144 h. Two 22 central composite designs and response surface methodology were adopted to derive a statistical model to measure the effect of SORR and CSL on the surface tension of the free-cell culture broth for 144 h. The regression equation obtained from the experimental data using a central composite design was solved, and by analyzing the response surface contour plots, the optimal concentrations of the constituents of the medium were determined: 8.63% v/v (≈9% v/v) of SORR and 8.80% v/v (≈9% v/v) CSL. The minimum surface tension predicted and experimentally confirmed was 25.25 mN/m. The new biosurfactant, denominated Lunasan, recovered 95% of motor oil adsorbed in a sand sample, thus showing great potential for use in bioremediation processes, especially in the petroleum industry.
Keywords: factorial design; central composite design; optimization; Candida sphaerica; tensio-active agent

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Luna, J.M.; Rufino, R.D.; Albuquerque, C.D.; Sarubbo, L.A.; Campos-Takaki, G.M. Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand. Int. J. Mol. Sci. 2011, 12, 2463-2476.

AMA Style

Luna JM, Rufino RD, Albuquerque CD, Sarubbo LA, Campos-Takaki GM. Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand. International Journal of Molecular Sciences. 2011; 12(4):2463-2476.

Chicago/Turabian Style

Luna, Juliana M.; Rufino, Raquel D.; Albuquerque, Clarissa D.C.; Sarubbo, Leonie A.; Campos-Takaki, Galba M. 2011. "Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand." Int. J. Mol. Sci. 12, no. 4: 2463-2476.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert