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Int. J. Mol. Sci. 2011, 12(4), 2315-2324; doi:10.3390/ijms12042315

Molecular Recognition of Arginine by Supramolecular Complexation with Calixarene Crown Ether Based on Surface Plasmon Resonance

1
School of Life Sciences, Shanghai University, Shanghai 200444, China
2
Henan Chemical Industry Research Institute Co. Ltd., Zhengzhou 450052, China
3
Department of Oncology, Jiangsu Province Hospital, Nanjing 210029, China
4
College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea
*
Author to whom correspondence should be addressed.
Received: 10 February 2011 / Revised: 16 March 2011 / Accepted: 17 March 2011 / Published: 4 April 2011
(This article belongs to the Section Molecular Recognition)
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Abstract

Arginine plays an important role in cell division and the functioning of the immune system. We describe a novel method by which arginine can be identified using an artificial monolayer based on surface plasmon resonance (SPR). The affinity of arginine binding its recognition molecular was compared to that of lysine. In fabrication of an arginine sensing interface, a calix[4]crown ether monolayer was anchored onto a gold surface and then characterized by Fourier Transform infrared reflection absorption spectroscopy, atomic force microscopy, and cyclic voltammetry. The interaction between arginine and its host compound was investigated by SPR. The calix[4]crown ether was found to assemble as a monolayer on the gold surface. Recognition of calix[4]crown monolayer was assessed by the selective binding of arginine. Modification of the SPR chip with the calix[4]crown monolayer provides a reliable and simple experimental platform for investigation of arginine under aqueous conditions.
Keywords: arginine; calixarene crown ether; amino acid; self-assembled monolayer (SAM); surface plasmon resonance (SPR) arginine; calixarene crown ether; amino acid; self-assembled monolayer (SAM); surface plasmon resonance (SPR)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chen, H.; Gu, L.; Yin, Y.; Koh, K.; Lee, J. Molecular Recognition of Arginine by Supramolecular Complexation with Calixarene Crown Ether Based on Surface Plasmon Resonance. Int. J. Mol. Sci. 2011, 12, 2315-2324.

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