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Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein
Department of Physics and CNISM, University of Parma, V.le Usberti 7A, Parma, Italy
Department of Animal Production, Veterinary Biotechnologies, Food Quality and Safety, University of Parma, V. del Taglio 8, Parma, Italy
Department of Biochemistry and Molecular Biology, University of Parma, V.le Usberti 23A, Parma, Italy
* Author to whom correspondence should be addressed.
Received: 1 March 2011; in revised form: 16 March 2011 / Accepted: 29 March 2011 / Published: 4 April 2011
Abstract: The stability and functionality of GCC-bOBP, a monomeric triple mutant of bovine odorant binding protein, was investigated, in the presence of denaturant and in acidic pH conditions, by both protein and 1-aminoanthracene ligand fluorescence measurements, and compared to that of both bovine and porcine wild type homologues. Complete reversibility of unfolding was observed, though refolding was characterized by hysteresis. Molecular dynamics simulations, performed to detect possible structural changes of the monomeric scaffold related to the presence of the ligand, pointed out the stability of the β-barrel lipocalin scaffold.
Keywords: odorant binding proteins; unfolding/refolding; molecular dynamics
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Polverini, E.; Lardi, P.; Mazzini, A.; Sorbi, R.T.; Virna, C.; Ramoni, R.; Favilla, R. Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein. Int. J. Mol. Sci. 2011, 12, 2294-2314.
Polverini E, Lardi P, Mazzini A, Sorbi RT, Virna C, Ramoni R, Favilla R. Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein. International Journal of Molecular Sciences. 2011; 12(4):2294-2314.
Polverini, Eugenia; Lardi, Paolo; Mazzini, Alberto; Sorbi, Robert T.; Virna, Conti; Ramoni, Roberto; Favilla, Roberto. 2011. "Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein." Int. J. Mol. Sci. 12, no. 4: 2294-2314.