Int. J. Mol. Sci. 2011, 12(4), 2294-2314; doi:10.3390/ijms12042294
Article

Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein

1 Department of Physics and CNISM, University of Parma, V.le Usberti 7A, Parma, Italy 2 Department of Animal Production, Veterinary Biotechnologies, Food Quality and Safety, University of Parma, V. del Taglio 8, Parma, Italy 3 Department of Biochemistry and Molecular Biology, University of Parma, V.le Usberti 23A, Parma, Italy
* Author to whom correspondence should be addressed.
Received: 1 March 2011; in revised form: 16 March 2011 / Accepted: 29 March 2011 / Published: 4 April 2011
(This article belongs to the Special Issue Protein Folding 2011)
PDF Full-text Download PDF Full-Text [725 KB, Updated Version, uploaded 6 April 2011 08:46 CEST]
The original version is still available [1331 KB, uploaded 4 April 2011 15:29 CEST]
Abstract: The stability and functionality of GCC-bOBP, a monomeric triple mutant of bovine odorant binding protein, was investigated, in the presence of denaturant and in acidic pH conditions, by both protein and 1-aminoanthracene ligand fluorescence measurements, and compared to that of both bovine and porcine wild type homologues. Complete reversibility of unfolding was observed, though refolding was characterized by hysteresis. Molecular dynamics simulations, performed to detect possible structural changes of the monomeric scaffold related to the presence of the ligand, pointed out the stability of the β-barrel lipocalin scaffold.
Keywords: odorant binding proteins; unfolding/refolding; molecular dynamics

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Polverini, E.; Lardi, P.; Mazzini, A.; Sorbi, R.T.; Virna, C.; Ramoni, R.; Favilla, R. Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein. Int. J. Mol. Sci. 2011, 12, 2294-2314.

AMA Style

Polverini E, Lardi P, Mazzini A, Sorbi RT, Virna C, Ramoni R, Favilla R. Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein. International Journal of Molecular Sciences. 2011; 12(4):2294-2314.

Chicago/Turabian Style

Polverini, Eugenia; Lardi, Paolo; Mazzini, Alberto; Sorbi, Robert T.; Virna, Conti; Ramoni, Roberto; Favilla, Roberto. 2011. "Characterization of a Deswapped Triple Mutant Bovine Odorant Binding Protein." Int. J. Mol. Sci. 12, no. 4: 2294-2314.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert