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Folding Mechanism of Beta-Hairpin Trpzip2: Heterogeneity, Transition State and Folding Pathways
Int. J. Mol. Sci. 2010, 11(10), 4014-4034; doi:10.3390/ijms11104014

Stability and Folding Behavior Analysis of Zinc-Finger Using Simple Models

1,* , 2
1 College of Informatics, South China Agricultural University, Guangzhou 510642, China 2 Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of technology, Taiyuan 030024, China 3 College of Chemistry and Life Science, Leshan Teacher's College, Leshan 614000, China
* Authors to whom correspondence should be addressed.
Received: 7 September 2010 / Revised: 1 October 2010 / Accepted: 9 October 2010 / Published: 19 October 2010
(This article belongs to the Special Issue Protein Folding 2011)
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Zinc-fingers play crucial roles in regulating gene expression and mediating protein-protein interactions. In this article, two different proteins (Sp1f2 and FSD-1) are investigated using the Gaussian network model and anisotropy elastic network model. By using these simple coarse-grained methods, we analyze the structural stabilization and establish the unfolding pathway of the two different proteins, in good agreement with related experimental and molecular dynamics simulation data. From the analysis, it is also found that the folding process of the zinc-finger motif is predominated by several factors. Both the zinc ion and C-terminal loop affect the folding pathway of the zinc-finger motif. Knowledge about the stability and folding behavior of zinc-fingers may help in understanding the folding mechanisms of the zinc-finger motif and in designing new zinc-fingers. Meanwhile, these simple coarse-grained analyses can be used as a general and quick method for mechanistic studies of metalloproteins.
Keywords: zinc finger; folding pathway; Gaussian network model; anisotropy elastic network model; Sp1f2; FSD-1 zinc finger; folding pathway; Gaussian network model; anisotropy elastic network model; Sp1f2; FSD-1
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chang, S.; Jiao, X.; Hu, J.-P.; Chen, Y.; Tian, X.-H. Stability and Folding Behavior Analysis of Zinc-Finger Using Simple Models. Int. J. Mol. Sci. 2010, 11, 4014-4034.

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