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Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour
Grain College, Henan University of Technology, Zhengzhou, 450052, China
Agriculture and Agri-Food Canada, Guelph Food Research Centre, Guelph, Ontario, N1G 5C9, Canada
* Author to whom correspondence should be addressed.
Received: 19 December 2010; in revised form: 16 February 2011 / Accepted: 18 February 2011 / Published: 28 February 2011
Abstract: Six hull-less barley cultivars widely grown in China were roller-milled to produce bran, shorts and flour fractions. The distribution and molecular characteristics of β-glucans from the three roller-milled fractions were investigated. The β-glucan contents in the six hull-less barley cultivars varied from 4.96% to 7.62%. For all the six cultivars, the shorts fraction contained the highest concentration of β-glucan (8.12–13.01%), followed by bran (6.15–7.58%) and flour (2.48–2.95%). Crude β-glucans were prepared from the three roller-milled fractions using aqueous sodium carbonate (pH 10). These preparations contained 45.38–71.41% β-glucan, 10.81–17.26% arabinoxylan, 2.6–9.6% protein, 2.7–9.0% starch, and 5.23–9.68% ash. Purification using a-amylase and β-xylanase in combination with pH adjustment and dialysis produced high purity β-glucan preparations (91–95%). The molecular weight (Mw) of β-glucan preparations from roller-milled fractions ranged from 117,600 to 852,400 g/mol. β-Glucan from flour had higher Mw than those from shorts and bran within the same cultivar, and β-glucan preparations from bran had the lowest Mw.
Keywords: β-glucan; molecular characterization; hull-less barley; flour; shorts; bran
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Zheng, X.; Li, L.; Wang, Q. Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour. Int. J. Mol. Sci. 2011, 12, 1563-1574.
Zheng X, Li L, Wang Q. Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour. International Journal of Molecular Sciences. 2011; 12(3):1563-1574.
Zheng, Xueling; Li, Limin; Wang, Qi. 2011. "Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour." Int. J. Mol. Sci. 12, no. 3: 1563-1574.