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Int. J. Mol. Sci. 2011, 12(3), 1563-1574; doi:10.3390/ijms12031563
Article

Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour

1,* , 1
 and 2
Received: 19 December 2010; in revised form: 16 February 2011 / Accepted: 18 February 2011 / Published: 28 February 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Six hull-less barley cultivars widely grown in China were roller-milled to produce bran, shorts and flour fractions. The distribution and molecular characteristics of β-glucans from the three roller-milled fractions were investigated. The β-glucan contents in the six hull-less barley cultivars varied from 4.96% to 7.62%. For all the six cultivars, the shorts fraction contained the highest concentration of β-glucan (8.12–13.01%), followed by bran (6.15–7.58%) and flour (2.48–2.95%). Crude β-glucans were prepared from the three roller-milled fractions using aqueous sodium carbonate (pH 10). These preparations contained 45.38–71.41% β-glucan, 10.81–17.26% arabinoxylan, 2.6–9.6% protein, 2.7–9.0% starch, and 5.23–9.68% ash. Purification using a-amylase and β-xylanase in combination with pH adjustment and dialysis produced high purity β-glucan preparations (91–95%). The molecular weight (Mw) of β-glucan preparations from roller-milled fractions ranged from 117,600 to 852,400 g/mol. β-Glucan from flour had higher Mw than those from shorts and bran within the same cultivar, and β-glucan preparations from bran had the lowest Mw.
Keywords: β-glucan; molecular characterization; hull-less barley; flour; shorts; bran β-glucan; molecular characterization; hull-less barley; flour; shorts; bran
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zheng, X.; Li, L.; Wang, Q. Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour. Int. J. Mol. Sci. 2011, 12, 1563-1574.

AMA Style

Zheng X, Li L, Wang Q. Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour. International Journal of Molecular Sciences. 2011; 12(3):1563-1574.

Chicago/Turabian Style

Zheng, Xueling; Li, Limin; Wang, Qi. 2011. "Distribution and Molecular Characterization of β-Glucans from Hull-Less Barley Bran, Shorts and Flour." Int. J. Mol. Sci. 12, no. 3: 1563-1574.


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