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Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China
* Author to whom correspondence should be addressed.
Received: 13 January 2011; in revised form: 30 January 2011 / Accepted: 7 February 2011 / Published: 15 February 2011
Abstract: The polymeric procyanidins extracted from Acacia confusa stem bark were fractionated with a step gradient of water, methanol and acetone on a Sephadex LH-20 column. The antioxidant activity of the collected fractions was investigated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing/antioxidant power (FRAP) assays. All fractions possessed potent antioxidant activity with the highest activity observed for fraction F9. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and reversed-phase high performance liquid chromatography (RP-HPLC) analyses suggested that the collected fractions consisted primarily of oligomeric and polymeric procyanidins, with different polymer ranges and most abundant polymer size. For each fraction, catechin and epicatechin were present as both terminal and extension units, and epicatechin was the major component in the extended chain. The mean degree of polymerization (mDP) of each fraction differed, ranging from 1.68 (fraction F2) to 17.31 (fraction F11). There was a relationship between antioxidant activity (IC50/DPPH and FRAP) and mDP (R2DPPH = 0.861, P = 0.006 and R2FRAP = 0.608, P = 0.038), respectively. However, the highest antioxidant activity of fraction (F9) was not coincident with the maximum mDP of fraction (F11).
Keywords: Acacia confusa; stem bark; polymeric procyanidins; fractionation; antioxidant activity; MALDI-TOF MS; RP-HPLC
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Wei, S.-D.; Zhou, H.-C.; Lin, Y.-M. Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa. Int. J. Mol. Sci. 2011, 12, 1146-1160.
Wei S-D, Zhou H-C, Lin Y-M. Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa. International Journal of Molecular Sciences. 2011; 12(2):1146-1160.
Wei, Shu-Dong; Zhou, Hai-Chao; Lin, Yi-Ming. 2011. "Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa." Int. J. Mol. Sci. 12, no. 2: 1146-1160.