Development and Characterization of 20 Microsatellite Markers for Chinese Black Sleeper, Bostrychus sinensis
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Isolation of Microsatellite Markers
3.2. Data Analysis
4. Conclusions
Acknowledgments
References
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Locus Genbank no. | Repeat motif | Primer sequence(5′-3′) | Ta (°C) | Size range (bp) | Na | Ho/He | P-value |
---|---|---|---|---|---|---|---|
BSD026 | (CA)61 | F: CATAAAAGACCCATTGTAACTGCT | 58 | 256–280 | 8 | 0.865/0.825 | 0.0505 |
JN806116 | R: CTGTAGCCCTCAGGAGCACATA | ||||||
BSD121 | (AC)19 | F: CGCACTGTCATCATAGCACTC | 58 | 131–175 | 8 | 0.444/0.760 | 0.0060 |
JN806117 | R: CCACCTGACAATGATTTAGTT | ||||||
BSD137 | (TG)23 | F: CTGACCTGGACTTCCCCTGG | 58 | 202–330 | 22 | 0.879/0.933 | 1.0000 |
JN806118 | R: CTGGGACAGGAGATGAGTTTT | ||||||
BSB006 | (TG)6C(GT)13 | F: TATTCTGTAATTACTGATATGTGCA | 60 | 172–222 | 9 | 0.742/0.843 | 0.2209 |
JN806119 | R: TACACAAGACCAAAAAGGTTAGGAA | ||||||
BSW045 | (CT)6…(AC)70 | F: ACTTTTTTCTCAATTTGGTTTCTAA | 52 | 128–220 | 14 | 0.833/0.867 | 0.9975 |
JN806120 | R: TGTGCTCAGGGGTACCGGGA | ||||||
BSW068 | (AC)16 | F: CTACAACAGCATCAGCCAACC | 58 | 113–133 | 7 | 0.684/0.766 | 0.3483 |
JN806121 | R: ACTCCCAAACACTGTCCAAGAAC | ||||||
BSSD14 | (TG)43 | F: ATTTAGCGAGGCTTTATGTT | 55 | 200–244 | 11 | 0.567/0.754 | 0.5198 |
JN806122 | R: GGCTGGCTTCCATCTTTTCT | ||||||
BSSD21 | (TG)38 | F: GATCCATTCTTAAAACACTCGTTAT | 55 | 267–313 | 9 | 0.774/0.833 | 0.6818 |
JN806123 | R: CAGGAGCAGTATCCAGACAAAA | ||||||
BSSW83 | (TG)16 | F: CCAGCAGCACCTGACACTCCAT | 58 | 144–156 | 8 | 0.452/0.808 | 0.0000 * |
JN806124 | R: TCCAGTGTTTGAAACTCCTGCC | ||||||
BSE020 | (GT)25 | F: GATTTTCAGAGCAGCAGCGTTGGC | 66 | 239–339 | 10 | 0.769/0.824 | 0.9217 |
JN806125 | R: CCACAAACGGAGCGTCCCAAATCT | ||||||
BSSW87 | (GT)35 | F: CGCACAGTTGACGCTTCCTTTA | 64 | 314–388 | 10 | 0.680/0.860 | 0.7399 |
JN806126 | R: GCCTCCCTGTCAGCCTTCACT | ||||||
BSSW89 | (GT)33 | F: TTGTAGCATTCCTTCTGCCTGT | 52 | 182–246 | 10 | 0.583/0.884 | 0.1589 |
JN806127 | R: CTCACTCCATCGGAATGTGTCTA | ||||||
BSD106 | (CA)20T(AC)9 | F: GAGATGAGCAACAGGTGAGTC | 56 | 338–388 | 10 | 0.667/0.842 | 0.5932 |
JN806128 | R: CTGGCAGAAGAGGATTGATGG | ||||||
BSD045 | (GT)35 | F: AAATGGATGTGTGAGAATGTGAGGCA | 62 | 258–384 | 10 | 0.467/0.720 | 0.4404 |
JN806129 | R: TGTGAACTCGAATGTGGGAGGTACT | ||||||
BSW115 | (GT)37C(TG)17 | F: TGTGATGTGTGTTTTGGGTGGTTA | 64 | 437–541 | 7 | 0.708/0.764 | 0.8613 |
JN806130 | R: TGTGTCCTCTGAAGTACCTGAAGC | ||||||
BSW053 | (AC)5… (CA)7… (AC)50 | F: TGCCCCCCAGATACCGACATTA | 66 | 308–394 | 17 | 0.889/0.912 | 0.9997 |
JN806131 | R: CGAGAGGTGAGCCAGGTTCAGGACT | ||||||
BSD125 | (CA)6CGCACG(CA)35 | F: CGCTTCAGTTCTGTGGAGGTA | 56 | 137–185 | 9 | 0.714/0.827 | 0.9570 |
JN806132 | R: CTGTCTGCCAAAGTTCCTGTTA | ||||||
BSC001 | (GT)48 | F: CTTGTTATGTCAAACCGTAGCCTTA | 56 | 341–401 | 15 | 0.615/0.909 | 0.4762 |
JN806133 | R: CCCTATCGTCCCCTGTAGACCG | ||||||
BSE008 | (TG)19 | F: GCTGCTCATAAACAATCACTTC | 58 | 138–154 | 6 | 0.656/0.734 | 0.7305 |
JN806134 | R:GTTGTCTGTAATCAGTGGCTCTA | ||||||
BSC002 | (ACT)18 | F: ATCAGCATCACAATGACCTGGGAG | 55 | 247–271 | 4 | 0.200/0.186 | 0.9926 |
JN806135 | R: CTTGGTGGAACTACAGACTTTTACA |
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Wang, H.-J.; Lin, H.-D.; Zhang, L.-Y.; Ding, S.-X. Development and Characterization of 20 Microsatellite Markers for Chinese Black Sleeper, Bostrychus sinensis. Int. J. Mol. Sci. 2011, 12, 9570-9575. https://doi.org/10.3390/ijms12129570
Wang H-J, Lin H-D, Zhang L-Y, Ding S-X. Development and Characterization of 20 Microsatellite Markers for Chinese Black Sleeper, Bostrychus sinensis. International Journal of Molecular Sciences. 2011; 12(12):9570-9575. https://doi.org/10.3390/ijms12129570
Chicago/Turabian StyleWang, Hang-Jun, Hung-Du Lin, Li-Yan Zhang, and Shao-Xiong Ding. 2011. "Development and Characterization of 20 Microsatellite Markers for Chinese Black Sleeper, Bostrychus sinensis" International Journal of Molecular Sciences 12, no. 12: 9570-9575. https://doi.org/10.3390/ijms12129570