RNA Aptamer Evolution: Two Decades of SELEction
Abstract
:1. Long Story Short, the Birth of Selection Methods
2. Setting the Ground for First Selections (1990–1994)
2.1. Classic SELEX
2.2. Negative-SELEX
2.3. Counter-SELEX
3. Improving the Libraries (1995–1996)
3.1. Blended SELEX
3.2. Photo-SELEX
3.3. cDNA-SELEX
3.4. Spiegelmer Technology
4. First Attempts to Enter the Cell Environment (1997–1999)
4.1. In Vivo SELEX
4.2. Chimeric SELEX
4.3. Multi Stage SELEX
5. Aptamer Regulation and Detection (2000–2003)
5.1. Signaling Aptamers
5.2. Indirect SELEX
5.3. Toggle SELEX
5.4. Expression Cassettes
5.5. Tailored SELEX
6. Updating SELEX Method with Modern Technologies (2004–2011)
6.1. CE-SELEX
6.2. FluMAG SELEX
6.3. TECS-SELEX
6.4. Non-SELEX
6.5. NanoSelection® (nM-AFM SELEX)
6.6. MonoLEX
6.7. CS-SELEX
6.8. Next Generation SELEX
6.9. Microfluidics SELEX (M-SELEX)
6.10. Multiple-Target High-Throughput SELEX
7. Bioinformatics Approaches for SELEX
8. Final Remarks
Acknowledgement
- Conflict of InterestThe authors declare no conflict of interest.
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Year | SELEX type | References * |
---|---|---|
1990–1993 | Classic, Negative | 1,2,5 |
1994 | Counter or Subtractive | 6,7 |
1995 | Blended (Covalent), Photoselex (crosslinked), cDNA-SELEX | 8–10 |
1996 | Spiegelmer isolation | 12 |
1997 | In vivo | 13 |
1998 | Chimeric | 14 |
1999 | Multistage, Cell Specific SELEX(CS-SELEX) | 15 |
2000 | Beacon aptamers, Indirect | 16–18 |
2001 | Toggle | 19,20 |
2002 | Expression cassette | 21 |
2003 | Tailored-SELEX | 22 |
2004 | CE-SELEX | 23 |
2005 | FluMAG | 24 |
2006 | TECS-SELEX, NON-SELEX (NCEEM) | 25,27 |
2007 | NanoSelection® (nM-AFM SELEX), MonoLEX | 28,30 |
2008 | CS-SELEX | 31,32 |
2009 | Next-generation SELEX | 33 |
2010 | Microfluidic-SELEX, Bioinformatics analyses | 36,37,43,44 |
2011 | Multiple-target high-throughput SELEX | 38–41 |
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Aquino-Jarquin, G.; Toscano-Garibay, J.D. RNA Aptamer Evolution: Two Decades of SELEction. Int. J. Mol. Sci. 2011, 12, 9155-9171. https://doi.org/10.3390/ijms12129155
Aquino-Jarquin G, Toscano-Garibay JD. RNA Aptamer Evolution: Two Decades of SELEction. International Journal of Molecular Sciences. 2011; 12(12):9155-9171. https://doi.org/10.3390/ijms12129155
Chicago/Turabian StyleAquino-Jarquin, Guillermo, and Julia D. Toscano-Garibay. 2011. "RNA Aptamer Evolution: Two Decades of SELEction" International Journal of Molecular Sciences 12, no. 12: 9155-9171. https://doi.org/10.3390/ijms12129155