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Int. J. Mol. Sci. 2011, 12(12), 8695-8712; doi:10.3390/ijms12128695
Article

Genetic Association and Altered Gene Expression of Mir-155 in Multiple Sclerosis Patients

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Received: 19 September 2011; in revised form: 31 October 2011 / Accepted: 22 November 2011 / Published: 1 December 2011
(This article belongs to the Special Issue Non-Coding RNAs)
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Abstract: Multiple sclerosis (MS) is a complex autoimmune disease of the central nervous system characterized by chronic inflammation, demyelination, and axonal damage. As microRNA (miRNA)-dependent alterations in gene expression in hematopoietic cells are critical for mounting an appropriate immune response, miRNA deregulation may result in defects in immune tolerance. In this frame, we sought to explore the possible involvement of miRNAs in MS pathogenesis by monitoring the differential expression of 22 immunity-related miRNAs in peripheral blood mononuclear cells of MS patients and healthy controls, by using a microbead-based technology. Three miRNAs resulted >2 folds up-regulated in MS vs controls, whereas none resulted down-regulated. Interestingly, the most up-regulated miRNA (mir-155; fold change = 3.30; P = 0.013) was previously reported to be up-regulated also in MS brain lesions. Mir-155 up-regulation was confirmed by qPCR experiments. The role of mir-155 in MS susceptibility was also investigated by genotyping four single nucleotide polymorphisms (SNPs) mapping in the mir-155 genomic region. A haplotype of three SNPs, corresponding to a 12-kb region encompassing the last exon of BIC (the B-cell Integration Cluster non-coding RNA, from which mir-155 is processed), resulted associated with the disease status (P = 0.035; OR = 1.36, 95% CI = 1.05–1.77), suggesting that this locus strongly deserves further investigations.
Keywords: multiple sclerosis; miRNA; expression profile; mir-155; association analysis multiple sclerosis; miRNA; expression profile; mir-155; association analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Paraboschi, E.M.; Soldà, G.; Gemmati, D.; Orioli, E.; Zeri, G.; Benedetti, M.D.; Salviati, A.; Barizzone, N.; Leone, M.; Duga, S.; Asselta, R. Genetic Association and Altered Gene Expression of Mir-155 in Multiple Sclerosis Patients. Int. J. Mol. Sci. 2011, 12, 8695-8712.

AMA Style

Paraboschi EM, Soldà G, Gemmati D, Orioli E, Zeri G, Benedetti MD, Salviati A, Barizzone N, Leone M, Duga S, Asselta R. Genetic Association and Altered Gene Expression of Mir-155 in Multiple Sclerosis Patients. International Journal of Molecular Sciences. 2011; 12(12):8695-8712.

Chicago/Turabian Style

Paraboschi, Elvezia Maria; Soldà, Giulia; Gemmati, Donato; Orioli, Elisa; Zeri, Giulia; Benedetti, Maria Donata; Salviati, Alessandro; Barizzone, Nadia; Leone, Maurizio; Duga, Stefano; Asselta, Rosanna. 2011. "Genetic Association and Altered Gene Expression of Mir-155 in Multiple Sclerosis Patients." Int. J. Mol. Sci. 12, no. 12: 8695-8712.


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