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Int. J. Mol. Sci. 2011, 12(12), 8982-8992; doi:10.3390/ijms12128982
Article

Lycium barbarum Polysaccharides Attenuate Cisplatin-Induced Hair Cell Loss in Rat Cochlear Organotypic Cultures

1,†
,
1,†
,
2
 and
1,*
Received: 29 September 2011 / Revised: 6 November 2011 / Accepted: 25 November 2011 / Published: 6 December 2011
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

The aim of the present study was to investigate the effects of Lycium barbarum polysaccharides (LBP) on cisplatin-induced hair cell damage in the organ of Corti explant. The neonatal (P2-3) rat organ of Corti explant was exposed to cisplatin (20 μM; 48 h) with or without LBP pretreatment (150 and 600 μg/mL; 24 h). Hair cell loss was indicated by FITC-labeled phalloidin staining. The level of reactive oxygen species (ROS) and alteration of mitochondrial membrane potential (ΔΨm) in hair cells were analyzed using fluorescent probes 2′,7′-dichlorofluorescein diacetate and JC-1, respectively. The results showed that LBP significantly attenuated hair cell loss (p < 0.01). Hair cells pretreated with LBP showed significant reduction in ROS production and the decline of ΔΨm compared with cisplatin alone group (p < 0.01), indicating the protective effect of LBP on cisplatin-induced hair cell loss. Taken together, these results indicate that LBP was effective in attenuating cisplatin-induced hair cell loss by reducing the production of ROS and maintaining mitochondrial ΔΨm.
Keywords: Lycium barbarum polysaccharides; cisplatin; hair cells; reactive oxygen species; mitochondria Lycium barbarum polysaccharides; cisplatin; hair cells; reactive oxygen species; mitochondria
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Liu, Q.; Li, Y.; Hu, L.; Wang, D. Lycium barbarum Polysaccharides Attenuate Cisplatin-Induced Hair Cell Loss in Rat Cochlear Organotypic Cultures. Int. J. Mol. Sci. 2011, 12, 8982-8992.

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