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Int. J. Mol. Sci. 2011, 12(12), 8982-8992; doi:10.3390/ijms12128982
Article

Lycium barbarum Polysaccharides Attenuate Cisplatin-Induced Hair Cell Loss in Rat Cochlear Organotypic Cultures

1,†
,
1,†
,
2
 and
1,*
1 Department of Otolaryngology, Eye, Ear, Nose and Throat hospital, Shanghai Medical College of Fudan University, Shanghai 200031, China 2 Central Laboratory, Eye, Ear, Nose and Throat hospital, Shanghai Medical College of Fudan University, Shanghai 200031, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 29 September 2011 / Revised: 6 November 2011 / Accepted: 25 November 2011 / Published: 6 December 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The aim of the present study was to investigate the effects of Lycium barbarum polysaccharides (LBP) on cisplatin-induced hair cell damage in the organ of Corti explant. The neonatal (P2-3) rat organ of Corti explant was exposed to cisplatin (20 μM; 48 h) with or without LBP pretreatment (150 and 600 μg/mL; 24 h). Hair cell loss was indicated by FITC-labeled phalloidin staining. The level of reactive oxygen species (ROS) and alteration of mitochondrial membrane potential (ΔΨm) in hair cells were analyzed using fluorescent probes 2′,7′-dichlorofluorescein diacetate and JC-1, respectively. The results showed that LBP significantly attenuated hair cell loss (p < 0.01). Hair cells pretreated with LBP showed significant reduction in ROS production and the decline of ΔΨm compared with cisplatin alone group (p < 0.01), indicating the protective effect of LBP on cisplatin-induced hair cell loss. Taken together, these results indicate that LBP was effective in attenuating cisplatin-induced hair cell loss by reducing the production of ROS and maintaining mitochondrial ΔΨm.
Keywords: Lycium barbarum polysaccharides; cisplatin; hair cells; reactive oxygen species; mitochondria Lycium barbarum polysaccharides; cisplatin; hair cells; reactive oxygen species; mitochondria
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Liu, Q.; Li, Y.; Hu, L.; Wang, D. Lycium barbarum Polysaccharides Attenuate Cisplatin-Induced Hair Cell Loss in Rat Cochlear Organotypic Cultures. Int. J. Mol. Sci. 2011, 12, 8982-8992.

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