Enhanced Antiproliferative and Apoptotic Response of HT-29 Adenocarcinoma Cells to Combination of Photoactivated Hypericin and Farnesyltransferase Inhibitor Manumycin A
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Cell Culture
3.2. Reagents
3.3. Experimental Design
3.4. Hypericin Treatment
3.5. Manumycin Treatment
3.6. Pan-Caspase Inhibitor Treatment
3.7. Hypericin Photoactivation
3.8. MTT Assay
3.9. Colony-Forming Assay
3.10. Cell Cycle Distribution Analysis
3.11. Caspase-3/7 Activity Assay
3.12. Western Blot Analysis
3.13. Cytosolic and Nuclear Fractionation
3.14. Antibodies
3.15. Immunofluorescent Staining of AIF
3.16. Statistical Analysis
4. Conclusions
Acknowledgments
- Conflict of interestThe authors declare no conflict of interest.
References
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Sačková, V.; Kuliková, L.; Kello, M.; Uhrinová, I.; Fedoročko, P. Enhanced Antiproliferative and Apoptotic Response of HT-29 Adenocarcinoma Cells to Combination of Photoactivated Hypericin and Farnesyltransferase Inhibitor Manumycin A. Int. J. Mol. Sci. 2011, 12, 8388-8405. https://doi.org/10.3390/ijms12128388
Sačková V, Kuliková L, Kello M, Uhrinová I, Fedoročko P. Enhanced Antiproliferative and Apoptotic Response of HT-29 Adenocarcinoma Cells to Combination of Photoactivated Hypericin and Farnesyltransferase Inhibitor Manumycin A. International Journal of Molecular Sciences. 2011; 12(12):8388-8405. https://doi.org/10.3390/ijms12128388
Chicago/Turabian StyleSačková, Veronika, Lucia Kuliková, Martin Kello, Ivana Uhrinová, and Peter Fedoročko. 2011. "Enhanced Antiproliferative and Apoptotic Response of HT-29 Adenocarcinoma Cells to Combination of Photoactivated Hypericin and Farnesyltransferase Inhibitor Manumycin A" International Journal of Molecular Sciences 12, no. 12: 8388-8405. https://doi.org/10.3390/ijms12128388