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Seasonal Variation in the Hepatoproteome of the Dehydration- and Freeze-Tolerant Wood Frog, Rana sylvatica
Laboratory for Ecophysiological Cryobiology, Department of Zoology, Miami University, Oxford, OH 45056, USA
Center for Bioinformatics and Functional Genomics, Miami University, Oxford, OH 45056, USA
Department of Biology, Augustana College, Rock Island, IL 61201, USA
* Author to whom correspondence should be addressed.
Received: 17 October 2011; in revised form: 12 November 2011 / Accepted: 21 November 2011 / Published: 29 November 2011
Abstract: Winter’s advent invokes physiological adjustments that permit temperate ectotherms to cope with stresses such as food shortage, water deprivation, hypoxia, and hypothermia. We used liquid chromatography (LC) in combination with tandem mass spectrometry (MS/MS) quantitative isobaric (iTRAQ™) peptide mapping to assess variation in the abundance of hepatic proteins in summer- and winter-acclimatized wood frogs (Rana sylvatica), a northerly-distributed species that tolerates extreme dehydration and tissue freezing during hibernation. Thirty-three unique proteins exhibited strong seasonal lability. Livers of winter frogs had relatively high levels of proteins involved in cytoprotection, including heat-shock proteins and an antioxidant, and a reduced abundance of proteins involved in cell proliferation, protein synthesis, and mitochondrial function. They also exhibited altered levels of certain metabolic enzymes that participate in the biochemical reorganization associated with aphagia and reliance on energy reserves, as well as the freezing mobilization and post-thaw recovery of glucose, an important cryoprotective solute in freezing adaptation.
Keywords: anuran; hibernation; LC-MS/MS; iTRAQ™; metabolism; seasonal variation
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Kiss, A.J.; Muir, T.J.; Lee, Jr, R.E.; Costanzo, J.P. Seasonal Variation in the Hepatoproteome of the Dehydration- and Freeze-Tolerant Wood Frog, Rana sylvatica. Int. J. Mol. Sci. 2011, 12, 8406-8414.
Kiss AJ, Muir TJ, Lee, Jr RE, Costanzo JP. Seasonal Variation in the Hepatoproteome of the Dehydration- and Freeze-Tolerant Wood Frog, Rana sylvatica. International Journal of Molecular Sciences. 2011; 12(12):8406-8414.
Kiss, Andor J.; Muir, Timothy J.; Lee, Jr, Richard E.; Costanzo, Jon P. 2011. "Seasonal Variation in the Hepatoproteome of the Dehydration- and Freeze-Tolerant Wood Frog, Rana sylvatica." Int. J. Mol. Sci. 12, no. 12: 8406-8414.