Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2011, 12(11), 8275-8287; doi:10.3390/ijms12118275
Review

Active Protein Aggregates Produced in Escherichia coli

1,2,*  and 1,2
Received: 12 October 2011; in revised form: 11 November 2011 / Accepted: 11 November 2011 / Published: 22 November 2011
(This article belongs to the Special Issue Protein Aggregation)
View Full-Text   |   Download PDF [557 KB, uploaded 19 June 2014]
Abstract: Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs formation represented a bottleneck in protein production as they were considered as deposits of inactive proteins. However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins can be prepared. Such active protein particles can be further used for the isolation of pure proteins or as whole active protein particles in various biomedical and other applications. Therefore interest in understanding the mechanisms of their formation as well as their properties is increasing.
Keywords: E. coli; active protein aggregates; inclusion bodies; IBs E. coli; active protein aggregates; inclusion bodies; IBs
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Peternel, Š.; Komel, R. Active Protein Aggregates Produced in Escherichia coli. Int. J. Mol. Sci. 2011, 12, 8275-8287.

AMA Style

Peternel Š, Komel R. Active Protein Aggregates Produced in Escherichia coli. International Journal of Molecular Sciences. 2011; 12(11):8275-8287.

Chicago/Turabian Style

Peternel, Špela; Komel, Radovan. 2011. "Active Protein Aggregates Produced in Escherichia coli." Int. J. Mol. Sci. 12, no. 11: 8275-8287.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert