Int. J. Mol. Sci. 2011, 12(11), 7692-7707; doi:10.3390/ijms12117692
Article

Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses

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Received: 4 September 2011; in revised form: 31 October 2011 / Accepted: 1 November 2011 / Published: 8 November 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In animals and recently in plants, heme oxygenase-1 (HO1) has been found to confer protection against a variety of oxidant-induced cell and tissue injuries. In this study, a wheat (Triticum aestivum) HO1 gene TaHO1 was cloned and sequenced. It encodes a polypeptide of 31.7 kD with a putative N-terminal plastid transit peptide. The amino acid sequence of TaHO1 was found to be 78% similar to that of maize HO1. Phylogenetic analysis revealed that TaHO1 clusters together with the HO1-like sequences in plants. The purified recombinant TaHO1 protein expressed in Escherichia coli was active in the conversion of heme to biliverdin IXa (BV), and showed that the Vmax was 8.8 U·mg−1 protein with an apparent Km value for hemin of 3.04 μM. The optimum Tm and pH were 35 °C and 7.4, respectively. The result of subcellular localization of TaHO1 showed that the putative transit peptide was sufficient for green fluorescent protein (GFP) to localize in chloroplast and implied that TaHO1 gene product is at least localized in the chloroplast. Moreover, we found that TaHO1 mRNA could be differentially induced by the well-known nitric oxide (NO) donor sodium nitroprusside (SNP), gibberellin acid (GA), abscisic acid (ABA), hydrogen peroxide (H2O2) and NaCl treatments. Therefore, the results suggested that TaHO1 might play an important role in abiotic stress responses.
Keywords: abiotic stress responses; carbon monoxide; heme oxygenase-1; prokaryotic expression in E. coli; wheat
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MDPI and ACS Style

Xu, D.-K.; Jin, Q.-J.; Xie, Y.-J.; Liu, Y.-H.; Lin, Y.-T.; Shen, W.-B.; Zhou, Y.-J. Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses. Int. J. Mol. Sci. 2011, 12, 7692-7707.

AMA Style

Xu D-K, Jin Q-J, Xie Y-J, Liu Y-H, Lin Y-T, Shen W-B, Zhou Y-J. Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses. International Journal of Molecular Sciences. 2011; 12(11):7692-7707.

Chicago/Turabian Style

Xu, Dao-kun; Jin, Qi-jiang; Xie, Yan-jie; Liu, Ya-hui; Lin, Yu-ting; Shen, Wen-biao; Zhou, Yi-jun. 2011. "Characterization of a Wheat Heme Oxygenase-1 Gene and Its Responses to Different Abiotic Stresses." Int. J. Mol. Sci. 12, no. 11: 7692-7707.

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