The Stimulation of IGF-1R Expression by Lewis(y) Antigen Provides a Powerful Development Mechanism of Epithelial Ovarian Carcinoma
Abstract
:Objective
Methods
Results
Conclusion
1. Introduction
2. Results
2.1. IGF-1R mRNA and Protein Expression in RMG-I and RMG-I-H Cells before and after Antibody Blocking
2.2. Expression of IGF-1R Protein and Lewis(y) on the Surface of RMG-I and RMG-I-H Cells and in Epithelial Ovarian Cancer Tissues
2.3. Expression of Lewis(y) and IGF-1R in Various Ovarian Tissues
2.4. Relationship between Expression of IGF-1R and Antigen Lewis(y), and Clinical Features of Ovarian Cancer
2.5. Staining Intensity of Lewis(y) and IGF-1R with Relation to Clinicopathological Parameters of Ovarian Cancer
3. Discussion
4. Material and Method
4.1. Reagents and Cells
4.2. Patients and Tissue Samples
4.3. Cell Culture
4.4. Determination of the Expression of IGF-1R mRNAs with Semi-Quantitative RT-PCR
4.5. Analysis of the Proteins of IGF-1R with Western Blotting
4.6. Analysis of Lewis(y) Expression on IGF-1R Using Immunoprecipitation and Western Blotting
4.7. Detection of Lewis(y) and IGF-1R Location on Cells and Tumor Tissue by Double-Labeling Immunofluorescence
4.8. Immunohistochemistry
4.9. Assessment Standard and Statistical Analysis
5. Conclusions
Acknowledgements
References
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Groups | Cases | Lewis(y) | Positive Cases | Rate (%) | IGF-1R | Positive Cases | Rate (%) | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
− | + | ++ | +++ | − | + | ++ | +++ | ||||||
Malignant group | 60 | 7 | 15 | 20 | 18 | 53 | 88.33 * | 4 | 10 | 23 | 23 | 56 | 93.33 * |
Borderline group | 30 | 12 | 6 | 11 | 1 | 18 | 60.00† | 11 | 9 | 7 | 3 | 19 | 63.33‡ |
Benign group | 30 | 20 | 6 | 4 | 0 | 10 | 33.33 | 14 | 9 | 6 | 1 | 16 | 53.33 |
Normal group | 20 | 20 | 0 | 0 | 0 | 0 | 00.00 | 12 | 6 | 2 | 0 | 8 | 40.00 |
Features | Case | Lewis(y) | IGF-1R | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Positive Cases | Rate (%) | P | MOD | P | Positive Cases | Rate (%) | P | MOD | P | |||
Pathological type | Mucous | 30 | 26 | 86.67 | >0.05 | 0.463 ± 0.068 | >0.05 | 24 | 80.00 | >0.05 | 0.418 ± 0.044 | >0.05 |
Serous | 30 | 27 | 90.00 | 0.477 ± 0.016 | 27 | 90.00 | 0.437 ± 0.051 | |||||
FIGO stage | I–II | 39 | 33 | 84.62 | >0.05 | 0.455 ± 0.065 | <0.05 | 33 | 84.62 | >0.05 | 0.413 ± 0.047 | <0.05 |
III–IV | 21 | 20 | 95.24 | 0.505 ± 0.072 | 18 | 85.71 | 0.444 ± 0.051 | |||||
Differentiation level | High | 21 | 17 | 80.95 | >0.05 | 0.448 ± 0.017 | <0.05 * | 16 | 76.19 | >0.05 | 0.412 ± 0.052 | <0.05 * |
Middle | 21 | 18 | 85.71 | 0.461 ± 0.054 | >0.05 † | 18 | 85.71 | 0.413 ± 0.038 | <0.05 † | |||
Low | 18 | 18 | 100 | 0.498 ± 0.084 | >0.05 ‡ | 17 | 94.44 | 0.455 ± 0.049 | >0.05 ‡ | |||
Lymphatic metastasis | No | 48 | 41 | 85.42 | >0.05 | 0.459 ± 0.078 | >0.05 | 40 | 83.33 | >0.05 | 0.420 ± 0.054 | >0.05 |
Yes | 12 | 12 | 100 | 0.476 ± 0.057 | 11 | 91.67 | 0.433 ± 0.060 |
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Liu, D.; Liu, J.; Wang, C.; Lin, B.; Liu, Q.; Hao, Y.; Zhang, S.; Iwamori, M. The Stimulation of IGF-1R Expression by Lewis(y) Antigen Provides a Powerful Development Mechanism of Epithelial Ovarian Carcinoma. Int. J. Mol. Sci. 2011, 12, 6781-6795. https://doi.org/10.3390/ijms12106781
Liu D, Liu J, Wang C, Lin B, Liu Q, Hao Y, Zhang S, Iwamori M. The Stimulation of IGF-1R Expression by Lewis(y) Antigen Provides a Powerful Development Mechanism of Epithelial Ovarian Carcinoma. International Journal of Molecular Sciences. 2011; 12(10):6781-6795. https://doi.org/10.3390/ijms12106781
Chicago/Turabian StyleLiu, Dawo, Juanjuan Liu, Changzhi Wang, Bei Lin, Qing Liu, Yingying Hao, Shulan Zhang, and Masao Iwamori. 2011. "The Stimulation of IGF-1R Expression by Lewis(y) Antigen Provides a Powerful Development Mechanism of Epithelial Ovarian Carcinoma" International Journal of Molecular Sciences 12, no. 10: 6781-6795. https://doi.org/10.3390/ijms12106781