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Int. J. Mol. Sci. 2011, 12(10), 6796-6809; doi:10.3390/ijms12106796
Article

Nuclear Markers of Danube Sturgeons Hybridization

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Received: 2 September 2011; in revised form: 27 September 2011 / Accepted: 8 October 2011 / Published: 14 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Acipenseriformes are composed of 25 sturgeon species and two paddlefish species distributed exclusively in the northern hemisphere. The Danube River and the Black Sea were originally inhabited by six sturgeon species but two are extinct and only four are still reproducing currently in the Lower Danube: Huso huso, Acipenser stellatus, A. gueldenstaedtii and A. ruthenus. Sturgeon species hybridize more easily than other fish and the determination of pure species or hybrid status is important for conservation and for breeding in fish farms. This survey demonstrated that morphological determination of this status is not reliable and a molecular tool, based on eight microsatellites genotypes is proposed. This method, based on three successive statistical analyses including Factorial Correspondence Analysis (FCA), Structure assignation and NewHybrids status determination, showed a high efficiency in discriminating pure species specimens from F1, F2 and two kinds of backcross individuals involving three of the four reproducing Lower Danube sturgeon species.
Keywords: sturgeons; microsatellites; hybrids; statistical analysis sturgeons; microsatellites; hybrids; statistical analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Dudu, A.; Suciu, R.; Paraschiv, M.; Georgescu, S.E.; Costache, M.; Berrebi, P. Nuclear Markers of Danube Sturgeons Hybridization. Int. J. Mol. Sci. 2011, 12, 6796-6809.

AMA Style

Dudu A, Suciu R, Paraschiv M, Georgescu SE, Costache M, Berrebi P. Nuclear Markers of Danube Sturgeons Hybridization. International Journal of Molecular Sciences. 2011; 12(10):6796-6809.

Chicago/Turabian Style

Dudu, Andreea; Suciu, Radu; Paraschiv, Marian; Georgescu, Sergiu Emil; Costache, Marieta; Berrebi, Patrick. 2011. "Nuclear Markers of Danube Sturgeons Hybridization." Int. J. Mol. Sci. 12, no. 10: 6796-6809.



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