Int. J. Mol. Sci. 2011, 12(10), 6749-6764; doi:10.3390/ijms12106749
Article

Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin

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Received: 1 August 2011; in revised form: 13 September 2011 / Accepted: 23 September 2011 / Published: 12 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype) were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.
Keywords: adipose derived stem cells; skin; adipose tissue; stem cells; Schwann cell; karyotypes
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MDPI and ACS Style

Gardin, C.; Vindigni, V.; Bressan, E.; Ferroni, L.; Nalesso, E.; Puppa, A.D.; D’Avella, D.; Lops, D.; Pinton, P.; Zavan, B. Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin. Int. J. Mol. Sci. 2011, 12, 6749-6764.

AMA Style

Gardin C, Vindigni V, Bressan E, Ferroni L, Nalesso E, Puppa AD, D’Avella D, Lops D, Pinton P, Zavan B. Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin. International Journal of Molecular Sciences. 2011; 12(10):6749-6764.

Chicago/Turabian Style

Gardin, Chiara; Vindigni, Vincenzo; Bressan, Eriberto; Ferroni, Letizia; Nalesso, Elisa; Puppa, Alessandro Della; D’Avella, Domenico; Lops, Diego; Pinton, Paolo; Zavan, Barbara. 2011. "Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin." Int. J. Mol. Sci. 12, no. 10: 6749-6764.


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