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Int. J. Mol. Sci. 2011, 12(10), 6749-6764; doi:10.3390/ijms12106749
Article

Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin

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1 Department of Histology, Microbiology and Medical Biotechnology, University of Padova, Via G. Colombo 3, 35100 Padova, Italy 2 Unit of Plastic and Reconstructive Surgery, University of Padova, Via Giustiniani 2, 35100 Padova, Italy 3 Department of Periodontology, School of Dentistry, University of Padova, Via Venezia 90, 35100 Padova, Italy 4 Unit of Neurosurgery, University of Padova, Via Giustiniani 2, 35100 Padova, Italy 5 Department of Prosthodontics, Dental Clinic, School of Dentistry, University of Milan, 21120 Milan, Italy 6 Department of Experimental and Diagnostic Medicine, Section of General Pathology, Interdisciplinary Center for the Study of Inflammation (ICSI) and LTTA center, University of Ferrara, 44100 Ferrara, Italy
* Author to whom correspondence should be addressed.
Received: 1 August 2011 / Revised: 13 September 2011 / Accepted: 23 September 2011 / Published: 12 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype) were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.
Keywords: adipose derived stem cells; skin; adipose tissue; stem cells; Schwann cell; karyotypes adipose derived stem cells; skin; adipose tissue; stem cells; Schwann cell; karyotypes
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Gardin, C.; Vindigni, V.; Bressan, E.; Ferroni, L.; Nalesso, E.; Puppa, A.D.; D’Avella, D.; Lops, D.; Pinton, P.; Zavan, B. Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin. Int. J. Mol. Sci. 2011, 12, 6749-6764.

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