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Int. J. Mol. Sci. 2011, 12(10), 6743-6748; doi:10.3390/ijms12106743

A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)

1,2
,
1,3
and
1,3,*
1
Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
2
Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
3
Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
*
Author to whom correspondence should be addressed.
Received: 7 September 2011 / Revised: 20 September 2011 / Accepted: 21 September 2011 / Published: 12 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Luculia gratissima (Wall.) Sweet (Rubiaceae) is a perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar. The plant is a distylous species with reciprocally placed stigmas and anthers in each floral morph. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing (FIASCO) repeats protocol, 19 primer sets were identified in two wild populations. Of these primers, 10 displayed polymorphisms and nine were monomorphic. The number of alleles per locus ranged from two to five, values for observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.289 to 0.760, with averages of 0.303 and 0.555, respectively. These microsatellite loci will facilitate further studies on breeding system, gene flow patterns, and population structure of L. gratissima and its allied species. View Full-Text
Keywords: Luculia gratissima; distylous; microsatellite marker; polymorphism; population genetics Luculia gratissima; distylous; microsatellite marker; polymorphism; population genetics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhou, W.; Li, D.; Wang, H. A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae). Int. J. Mol. Sci. 2011, 12, 6743-6748.

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