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Int. J. Mol. Sci. 2011, 12(10), 6445-6451; doi:10.3390/ijms12106445

A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)

Institute of Crop Science, Chinese Academy of Agricultural Sciences, South Xueyuan Road, Haidian District No.80, Beijing 100081, China
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Received: 12 July 2011 / Revised: 1 September 2011 / Accepted: 21 September 2011 / Published: 28 September 2011
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and α-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 0.4 mg·mL−1 and 4.8 mg·mL−1, respectively. This is the first study of the active compositions of azuki beans against α-glucosidase. View Full-Text
Keywords: azuki beans; α-glucosidase inhibitory; vitexin; isovitexin azuki beans; α-glucosidase inhibitory; vitexin; isovitexin
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Yao, Y.; Cheng, X.; Wang, L.; Wang, S.; Ren, G. A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis). Int. J. Mol. Sci. 2011, 12, 6445-6451.

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