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Int. J. Mol. Sci. 2011, 12(10), 6445-6451; doi:10.3390/ijms12106445

A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)

Institute of Crop Science, Chinese Academy of Agricultural Sciences, South Xueyuan Road, Haidian District No.80, Beijing 100081, China
* Author to whom correspondence should be addressed.
Received: 12 July 2011 / Revised: 1 September 2011 / Accepted: 21 September 2011 / Published: 28 September 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and α-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 0.4 mg·mL−1 and 4.8 mg·mL−1, respectively. This is the first study of the active compositions of azuki beans against α-glucosidase.
Keywords: azuki beans; α-glucosidase inhibitory; vitexin; isovitexin azuki beans; α-glucosidase inhibitory; vitexin; isovitexin
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yao, Y.; Cheng, X.; Wang, L.; Wang, S.; Ren, G. A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis). Int. J. Mol. Sci. 2011, 12, 6445-6451.

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