Abstract: A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and α-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 0.4 mg·mL−1 and 4.8 mg·mL−1, respectively. This is the first study of the active compositions of azuki beans against α-glucosidase.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Yao, Y.; Cheng, X.; Wang, L.; Wang, S.; Ren, G. A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis). Int. J. Mol. Sci. 2011, 12, 6445-6451.
Yao Y, Cheng X, Wang L, Wang S, Ren G. A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis). International Journal of Molecular Sciences. 2011; 12(10):6445-6451.
Yao, Yang; Cheng, Xuzhen; Wang, Lixia; Wang, Suhua; Ren, Guixing. 2011. "A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)." Int. J. Mol. Sci. 12, no. 10: 6445-6451.