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Int. J. Mol. Sci. 2011, 12(10), 6407-6417; doi:10.3390/ijms12106407
Article

Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin

1
,
1
,
1
,
1,2
 and
1,3,*
1 Institute of Food Nutrition and Human Health, Massey University, Palmerston North 4474, New Zealand 2 Department of Applied Science, London South Bank University, 103 Borough Road, London SE1 0AA, UK 3 Department of Wine and Molecular Biosciences, Lincoln University, Lincoln 7647, New Zealand
* Author to whom correspondence should be addressed.
Received: 1 August 2011 / Revised: 15 September 2011 / Accepted: 15 September 2011 / Published: 27 September 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The effects of Celluclast 1.5L concentration on the physicochemical characterization of gold kiwifruit pectin was evaluated. Varying the enzyme concentration affected the pectin yield and pectin physicochemical properties. The viscosity of extracted pectin was largely dependent on the enzyme concentration. Celluclast 1.5L with medium concentration exhibited the highest viscosity. Varying the enzyme concentration also influenced the molecular weight distribution. High molecular weight (Mw) pectin (1.65 × 106 g/mol) was obtained when the medium concentration was used. Overall, the study clearly reflects the importance of taking into consideration the amount of cellulytic enzyme added in order to determine the final quality of pectin.
Keywords: pectin; gold kiwifruit; pectin extraction; enzyme concentration; molecular weight; galacturonic acid; viscosity pectin; gold kiwifruit; pectin extraction; enzyme concentration; molecular weight; galacturonic acid; viscosity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yuliarti, O.; Matia-Merino, L.; Goh, K.K.T.; Mawson, J.A.; Brennan, C.S. Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin. Int. J. Mol. Sci. 2011, 12, 6407-6417.

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