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Int. J. Mol. Sci. 2010, 11(9), 3529-3539; doi:10.3390/ijms11093529

Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications

1,2,* , 1,3, 1,2, 1,2, 1,2 and 1,2
1 Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai, 201620, China 2 Biomaterials and Tissue Engineering Lab, College of Chemistry and Chemical Engineering and Biological Engineering, Donghua University, Shanghai, 201620, China 3 College of Biological Engineering and Chemical Engineering, Jiaxing College, Zhejiang, 314001, China
* Author to whom correspondence should be addressed.
Received: 24 August 2010 / Revised: 12 September 2010 / Accepted: 14 September 2010 / Published: 21 September 2010
(This article belongs to the Special Issue Chitins)
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Chitosan, a naturally occurring polysaccharide with abundant resources, has been extensively exploited for various biomedical applications, typically as wound dressings owing to its unique biocompatibility, good biodegradability and excellent antibacterial properties. In this work, composite nanofibrous membranes of chitosan (CS) and silk fibroin (SF) were successfully fabricated by electrospinning. The morphology of electrospun blend nanofibers was observed by scanning electron microscopy (SEM) and the fiber diameters decreased with the increasing percentage of chitosan. Further, the mechanical test illustrated that the addition of silk fibroin enhanced the mechanical properties of CS/SF nanofibers. The antibacterial activities against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) were evaluated by the turbidity measurement method; and results suggest that the antibacterial effect of composite nanofibers varied on the type of bacteria. Furthermore, the biocompatibility of murine fibroblast on as-prepared nanofibrous membranes was investigated by hematoxylin and eosin (H&E) staining and MTT assays in vitro, and the membranes were found to promote the cell attachment and proliferation. These results suggest that as-prepared chitosan/silk fibroin (CS/SF) composite nanofibrous membranes could be a promising candidate for wound healing applications.
Keywords: electrospinning; chitosan; composite nanofibers; antibacterial activity; wound dressing electrospinning; chitosan; composite nanofibers; antibacterial activity; wound dressing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cai, Z.-X.; Mo, X.-M.; Zhang, K.-H.; Fan, L.-P.; Yin, A.-L.; He, C.-L.; Wang, H.-S. Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications. Int. J. Mol. Sci. 2010, 11, 3529-3539.

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