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Int. J. Mol. Sci. 2010, 11(9), 3323-3333; doi:10.3390/ijms11093323
Article

The Mechanism of (R,R) ZX-5 on Increasing NO Release

1
, 1
, 1
, 1
, 1
, 2
 and 1,*
1 Department of Marine Pharmacy, College of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China 2 Department of Pharmacy, University of China Pharmaceutical, Nanjing 210009, Jiangsu Province, China
* Author to whom correspondence should be addressed.
Received: 18 August 2010 / Revised: 2 September 2010 / Accepted: 6 September 2010 / Published: 15 September 2010
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

(R,R) ZX-5 has been proven to have positive effects on choroidal blood flow without affecting the sclera and ciliary bodies in New Zealand white rabbits. This study was designed to investigate the mechanisms of (R,R) ZX-5 on improving the choroidal blood flow and promoting NO production. HUVECs (human umbilical vein endothelial cells) were used to determine the production of eNOS, p-eNOS, AKT and Erk1/2 by Western blot analysis. iNOS and eNOS mRNA levels were investigated by RT-PCR and the effect of (R,R) ZX-5 on NO production were determined by eNOS activity assay. We found (R,R) ZX-5 upregulated protein expression of eNOS and iNOS, increased NO production, and reduced ERK and Akt protein level. Therefore, (R,R) ZX-5 may promote the choroidal blood flow in New Zealand white rabbits without affecting the blood flow in the iris or ciliary bodies via increasing NO production. These results suggest that (R,R) ZX-5 may function to cure and prevent Age-related macular degeneration (AMD).
Keywords: (R,R) ZX-5; iNOS; eNOS; ERK; Akt (R,R) ZX-5; iNOS; eNOS; ERK; Akt
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Xu, H.-M.; Wei, J.; Pan, L.; Lin, H.; Wang, W.; Zhang, Y.; Shen, Z. The Mechanism of (R,R) ZX-5 on Increasing NO Release. Int. J. Mol. Sci. 2010, 11, 3323-3333.

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