Int. J. Mol. Sci. 2010, 11(7), 2670-2680; doi:10.3390/ijms11072670
Article

Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India

Muthuraj Muthaiah 1,* email, Sridharan Jagadeesan 1 , Nisha Ayalusamy 1 , Manupriya Sreenivasan 1 , Sambamurthy Sangamesvara Prabhu 1 , Usharani Muthuraj 2 , Kamatchiyammal Senthilkumar 3  and Saroja Veerappan 3
1 State TB Training and Demonstration Centre, Govt. Hospital for Chest Diseases, Puducherry, India 2 Departments of Microbiology, Vector Control Research Centre, Puducherry, India 3 Virology Cell, NEERI, CSIR Complex, Tharamani, Chennai, Tamil Nadu, India
* Author to whom correspondence should be addressed.
Received: 18 May 2010 / Accepted: 24 May 2010 / Published: 7 July 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Pyrazinamide (PZA) has been in use for almost 50 years as a first-line drug for short-course chemotherapy against Mycobacterium tuberculosis. In this study, PCR mediated automated DNA sequencing is used to check the prevalence of PZA resistance among treatment failure cases of pulmonary tuberculosis. Out of 50 clinical isolates examined, 39 had mutations in the pncA gene that encodes Pyrazinamidase, an enzyme required to activate PZA. Of these, 31 (79.5%) were localized to three regions of pncA. We found two isolates with hitherto unreported mutation at amino acid 26 (Ala→Gly) of pncA.
Keywords: PZA resistance; mycobacterium tuberculosis; pulmonary tuberculosis; South India; pncA mutation

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MDPI and ACS Style

Muthaiah, M.; Jagadeesan, S.; Ayalusamy, N.; Sreenivasan, M.; Prabhu, S.S.; Muthuraj, U.; Senthilkumar, K.; Veerappan, S. Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India. Int. J. Mol. Sci. 2010, 11, 2670-2680.

AMA Style

Muthaiah M, Jagadeesan S, Ayalusamy N, Sreenivasan M, Prabhu SS, Muthuraj U, Senthilkumar K, Veerappan S. Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India. International Journal of Molecular Sciences. 2010; 11(7):2670-2680.

Chicago/Turabian Style

Muthaiah, Muthuraj; Jagadeesan, Sridharan; Ayalusamy, Nisha; Sreenivasan, Manupriya; Prabhu, Sambamurthy Sangamesvara; Muthuraj, Usharani; Senthilkumar, Kamatchiyammal; Veerappan, Saroja. 2010. "Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India." Int. J. Mol. Sci. 11, no. 7: 2670-2680.

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