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Int. J. Mol. Sci. 2010, 11(7), 2612-2623; doi:10.3390/ijms11072612
Article

Antibiotic Producing Potentials of Three Freshwater Actinomycetes Isolated from the Eastern Cape Province of South Africa

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1 Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa 2 Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria 3 South African Environmental Observation Network (SAEON), Elwandle Node, Grahamstown, South Africa 4 Division of Microbiology, University of KwaZulu Natal, Durban Westville, South Africa
* Author to whom correspondence should be addressed.
Received: 10 June 2010 / Revised: 25 June 2010 / Accepted: 28 June 2010 / Published: 2 July 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Crude extracts of three actinomycetes species belonging to Saccharopolyspora (TR 046 and TR 039) and Actinosynnema (TR 024) genera were screened for antibacterial activities against a panel of several bacterial strains. The extracts showed antibacterial activities against both gram-negative and gram-positive test bacteria with inhibition zones ranging from 8 to 28 mm (TR 046); 8 to15 mm (TR 039); and 10 to 13 mm (TR 024). The minimum inhibitory concentrations ranged from 0.078 to 10 mg/mL (TR 046); 5 to >10 mg/mL (TR 039); and 1.25 to 5 mg/mL (TR 024). Time-kill studies revealed that crude extract of TR 046 showed strong bactericidal activity against Bacillus pumilus (ATCC14884), reducing the bacterial load by 104 cfu/mL and 102 cfu/mL at 4× MIC and 2× MIC, respectively, after 6 h of exposure. Similarly, against Proteus vulgaris (CSIR 0030), crude extract of TR 046 achieved a 0.9log10 and 0.13log10 cfu/mL reduction at 5 mg/mL (4× MIC) and 1.25 mg/mL (2× MIC) after 12 h of exposure. The extract was however weakly bactericidal against two environmental bacterial strains (Klebsiella pneumoniae and Staphylococcus epidermidis); and against Pseudomonas aeruginosa (ATCC 19582): the extract showed bacteriostatic activities at all concentrations tested. These freshwater actinomycetes appear to have immense potential as a source of new antibacterial compound(s).
Keywords: actinomycetes; crude extract; antibacterial activity; time-kill actinomycetes; crude extract; antibacterial activity; time-kill
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Sibanda, T.; Mabinya, L.V.; Mazomba, N.; Akinpelu, D.A.; Bernard, K.; Olaniran, A.O.; Okoh, A.I. Antibiotic Producing Potentials of Three Freshwater Actinomycetes Isolated from the Eastern Cape Province of South Africa. Int. J. Mol. Sci. 2010, 11, 2612-2623.

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