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Effect of Sodium Fluoride Ingestion on Malondialdehyde Concentration and the Activity of Antioxidant Enzymes in Rat Erythrocytes
Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, 42080 Pachuca de Soto, Hgo, Mexico
Laboratorio de Bioquímica y Medicina Experimental, División de Investigación Biomédica, Centro Médico Nacional 20 de Noviembre, ISSSTE, Mexico, D.F., Mexico
Laboratorio de Medicina Genómica, División de Medicina Genómica, Centro Médico Nacional 20 de Noviembre, ISSSTE, Mexico, D.F., Mexico
Facultad de Odontologia, Universidad Nacional Autónoma de México (UNAM), Mexico, D.F., Mexico
* Author to whom correspondence should be addressed.
Received: 27 April 2010; in revised form: 2 June 2010 / Accepted: 2 June 2010 / Published: 11 June 2010
Abstract: Fluoride intoxication has been shown to produce diverse deleterious metabolic alterations within the cell. To determine the effects of sodium fluoride (NaF) treatment on malondialdehyde (MDA) levels and on the activity of antioxidant enzymes in rat erythrocytes, Male Wistar rats were treated with 50 ppm of NaF or were untreated as controls. Erythrocytes were obtained from rats sacrificed weekly for up to eight weeks and the concentration of MDA in erythrocyte membrane was determined. In addition, the activity of the enzymes superoxide, dismutase, catalase, and glutathione peroxidase were determined. Treatment with NaF produces an increase in the concentration of malondialdehyde in the erythrocyte membrane only after the eight weeks of treatment. On the other hand, antioxidant enzyme activity was observed to increase after the fourth week of NaF treatment. In conclusion, intake of NaF produces alterations in the erythrocyte of the male rat, which indicates induction of oxidative stress.
Keywords: antioxidant enzymes; erythrocyte; oxidative stress; sodium fluoride; rat
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Morales-González, J.A.; Gutiérrez-Salinas, J.; García-Ortiz, L.; Del Carmen Chima-Galán, M.; Madrigal-Santillán, E.; Esquivel-Soto, J.; Esquivel-Chirino, C.; García-Luna y González-Rubio, M. Effect of Sodium Fluoride Ingestion on Malondialdehyde Concentration and the Activity of Antioxidant Enzymes in Rat Erythrocytes. Int. J. Mol. Sci. 2010, 11, 2443-2452.
Morales-González JA, Gutiérrez-Salinas J, García-Ortiz L, Del Carmen Chima-Galán M, Madrigal-Santillán E, Esquivel-Soto J, Esquivel-Chirino C, García-Luna y González-Rubio M. Effect of Sodium Fluoride Ingestion on Malondialdehyde Concentration and the Activity of Antioxidant Enzymes in Rat Erythrocytes. International Journal of Molecular Sciences. 2010; 11(6):2443-2452.
Morales-González, José A.; Gutiérrez-Salinas, José; García-Ortiz, Liliana; Del Carmen Chima-Galán, María; Madrigal-Santillán, Eduardo; Esquivel-Soto, Jaime; Esquivel-Chirino, César; García-Luna y González-Rubio, Manuel. 2010. "Effect of Sodium Fluoride Ingestion on Malondialdehyde Concentration and the Activity of Antioxidant Enzymes in Rat Erythrocytes." Int. J. Mol. Sci. 11, no. 6: 2443-2452.