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Int. J. Mol. Sci. 2010, 11(5), 2229-2240; doi:10.3390/ijms11052229
Article

Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC

,
 and *
Department of Chemical Engineering, Inha University, Incheon 402-751, Korea
* Author to whom correspondence should be addressed.
Received: 17 March 2010 / Revised: 28 April 2010 / Accepted: 21 May 2010 / Published: 25 May 2010
(This article belongs to the Section Green Chemistry)
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Abstract

The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C18 column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The result revealed that a mobile phase with 0.01 mol/L of ionic liquid [BMIM][BF4] enabled the optimum separation of the four target compounds.
Keywords: mobile phase additive; bioactive compounds; ionic liquids; Herba Artemisiae Scopariae mobile phase additive; bioactive compounds; ionic liquids; Herba Artemisiae Scopariae
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, S.; Tian, M.; Row, K.H. Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC. Int. J. Mol. Sci. 2010, 11, 2229-2240.

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