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β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds
Institute of Chemical Process Fundamentals of the AS CR, v.v.i., Rozvojová 135/2, 165 00 Prague 6, Czech Republic
Institute of Chemical Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic
Institute of Experimental Botany of the AS CR, v.v.i., Isotope Laboratory, Vídeňská 1083, 142 20 Prague 4, Czech Republic
* Author to whom correspondence should be addressed.
Received: 3 February 2010 / Accepted: 20 April 2010 / Published: 22 April 2010
Abstract: Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40-80 °C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 °C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 °C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide.
Keywords: Hippophae rhamnoides L.; sea buckthorn seed; fractionation in time; supercritical carbon dioxide; β-sitosterol
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Sajfrtová, M.; Ličková, I.; Wimmerová, M.; Sovová, H.; Wimmer, Z. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds. Int. J. Mol. Sci. 2010, 11, 1842-1850.
Sajfrtová M, Ličková I, Wimmerová M, Sovová H, Wimmer Z. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds. International Journal of Molecular Sciences. 2010; 11(4):1842-1850.
Sajfrtová, Marie; Ličková, Ivana; Wimmerová, Martina; Sovová, Helena; Wimmer, Zdeněk. 2010. "β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds." Int. J. Mol. Sci. 11, no. 4: 1842-1850.