Int. J. Mol. Sci. 2010, 11(4), 1842-1850; doi:10.3390/ijms11041842
Article

β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds

1email, 1,2email, 3email, 1email and 3,* email
Received: 3 February 2010; Accepted: 20 April 2010 / Published: 22 April 2010
(This article belongs to the Special Issue Supercritical Carbon Dioxide)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40-80 °C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 °C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 °C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide.
Keywords: Hippophae rhamnoides L.; sea buckthorn seed; fractionation in time; supercritical carbon dioxide; β-sitosterol
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MDPI and ACS Style

Sajfrtová, M.; Ličková, I.; Wimmerová, M.; Sovová, H.; Wimmer, Z. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds. Int. J. Mol. Sci. 2010, 11, 1842-1850.

AMA Style

Sajfrtová M, Ličková I, Wimmerová M, Sovová H, Wimmer Z. β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds. International Journal of Molecular Sciences. 2010; 11(4):1842-1850.

Chicago/Turabian Style

Sajfrtová, Marie; Ličková, Ivana; Wimmerová, Martina; Sovová, Helena; Wimmer, Zdeněk. 2010. "β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds." Int. J. Mol. Sci. 11, no. 4: 1842-1850.

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