Int. J. Mol. Sci. 2010, 11(4), 1458-1470; doi:10.3390/ijms11041458
Article

Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1

Seicho Makihira 1,* email, Takahiro Shuto 1 email, Hiroki Nikawa 1 email, Keishi Okamoto 2 email, Yuichi Mine 1 email, Yuko Takamoto 1 email, Masaru Ohara 3 email and Koichiro Tsuji 4 email
1 Division of Oral Health Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan 2 Toyo Advanced Technologies Co., LTD. 5-3-38, Ujina-Higashi, Minami-ku, Hiroshima 734-8501, Japan 3 Hiroshima University Hospital, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan 4 Two cell Co. LTD., 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan
* Author to whom correspondence should be addressed.
Received: 23 February 2010; in revised form: 23 March 2010 / Accepted: 23 March 2010 / Published: 2 April 2010
(This article belongs to the Special Issue Dental Materials)
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Abstract: The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.
Keywords: antimicrobial peptide; titanium surface; MC3T3-E1 cells

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MDPI and ACS Style

Makihira, S.; Shuto, T.; Nikawa, H.; Okamoto, K.; Mine, Y.; Takamoto, Y.; Ohara, M.; Tsuji, K. Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1. Int. J. Mol. Sci. 2010, 11, 1458-1470.

AMA Style

Makihira S, Shuto T, Nikawa H, Okamoto K, Mine Y, Takamoto Y, Ohara M, Tsuji K. Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1. International Journal of Molecular Sciences. 2010; 11(4):1458-1470.

Chicago/Turabian Style

Makihira, Seicho; Shuto, Takahiro; Nikawa, Hiroki; Okamoto, Keishi; Mine, Yuichi; Takamoto, Yuko; Ohara, Masaru; Tsuji, Koichiro. 2010. "Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1." Int. J. Mol. Sci. 11, no. 4: 1458-1470.

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