Int. J. Mol. Sci. 2010, 11(4), 1423-1433; doi:10.3390/ijms11041423
Article

Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry

Yiyi Sun 1 email, Zhihe Zang 1 , Xiaohong Xu 1 email, Zhonglin Zhang 1 email, Ling Zhong 1 email, Wang Zan 1 email, Yan Zhao 1 email and Lin Sun 2,* email
1 Chengdu Medical College, Chengdu 610083, Sichuan Province, China 2 West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
* Author to whom correspondence should be addressed.
Received: 11 January 2010; in revised form: 14 March 2010 / Accepted: 17 March 2010 / Published: 31 March 2010
(This article belongs to the Special Issue Biomarkers)
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Abstract: The aim of the current study is to identify the potential biomarkers involved in Hepatocellular carcinoma (HCC) carcinogenesis. A comparative proteomics approach was utilized to identify the differentially expressed proteins in the serum of 10 HCC patients and 10 controls. A total of 12 significantly altered proteins were identified by mass spectrometry. Of the 12 proteins identified, HSP90 was one of the most significantly altered proteins and its over-expression in the serum of 20 HCC patients was confirmed using ELISA analysis. The observations suggest that HSP90 might be a potential biomarker for early diagnosis, prognosis, and monitoring in the therapy of HCC. This work demonstrates that a comprehensive strategy of proteomic identification combined with further validation should be adopted in the field of cancer biomarker discovery.
Keywords: proteomics; hepatocellular carcinoma; serum biomarker

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Cite This Article

MDPI and ACS Style

Sun, Y.; Zang, Z.; Xu, X.; Zhang, Z.; Zhong, L.; Zan, W.; Zhao, Y.; Sun, L. Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry. Int. J. Mol. Sci. 2010, 11, 1423-1433.

AMA Style

Sun Y, Zang Z, Xu X, Zhang Z, Zhong L, Zan W, Zhao Y, Sun L. Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry. International Journal of Molecular Sciences. 2010; 11(4):1423-1433.

Chicago/Turabian Style

Sun, Yiyi; Zang, Zhihe; Xu, Xiaohong; Zhang, Zhonglin; Zhong, Ling; Zan, Wang; Zhao, Yan; Sun, Lin. 2010. "Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry." Int. J. Mol. Sci. 11, no. 4: 1423-1433.

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