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Int. J. Mol. Sci. 2010, 11(4), 1423-1433; doi:10.3390/ijms11041423
Article

Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry

1
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1, 1
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1
 and
2,*
1 Chengdu Medical College, Chengdu 610083, Sichuan Province, China 2 West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
* Author to whom correspondence should be addressed.
Received: 11 January 2010 / Revised: 14 March 2010 / Accepted: 17 March 2010 / Published: 31 March 2010
(This article belongs to the Special Issue Biomarkers)
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Abstract

The aim of the current study is to identify the potential biomarkers involved in Hepatocellular carcinoma (HCC) carcinogenesis. A comparative proteomics approach was utilized to identify the differentially expressed proteins in the serum of 10 HCC patients and 10 controls. A total of 12 significantly altered proteins were identified by mass spectrometry. Of the 12 proteins identified, HSP90 was one of the most significantly altered proteins and its over-expression in the serum of 20 HCC patients was confirmed using ELISA analysis. The observations suggest that HSP90 might be a potential biomarker for early diagnosis, prognosis, and monitoring in the therapy of HCC. This work demonstrates that a comprehensive strategy of proteomic identification combined with further validation should be adopted in the field of cancer biomarker discovery.
Keywords: proteomics; hepatocellular carcinoma; serum biomarker proteomics; hepatocellular carcinoma; serum biomarker
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Sun, Y.; Zang, Z.; Xu, X.; Zhang, Z.; Zhong, L.; Zan, W.; Zhao, Y.; Sun, L. Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry. Int. J. Mol. Sci. 2010, 11, 1423-1433.

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