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Miniaturization in Biocatalysis
Int. J. Mol. Sci. 2010, 11(3), 1180-1189; doi:10.3390/ijms11031180

Fructose Production by Inulinase Covalently Immobilized on Sepabeads in Batch and Fluidized Bed Bioreactor

1,* , 2
1 Department of Engineering Modeling, University of Calabria, I-87036 Arcavacata di Rende, CS, Italy 2 SPRIN S.p.A., c/o BIC, Via Flavia 23/A, 34100 Trieste, Italy 3 Laboratory of Applied and Computational Biocatalysis, Dipartimento di Scienze Farmaceutiche, Università degli Studi, P.le Europa 1, 34127 Trieste, Italy
* Author to whom correspondence should be addressed.
Received: 13 January 2010 / Accepted: 17 March 2010 / Published: 19 March 2010
(This article belongs to the Special Issue Biocatalysis)
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The present work is an experimental study of the performance of a recently designed immobilized enzyme: inulinase from Aspergillus sp. covalently immobilized on Sepabeads. The aim of the work is to test the new biocatalyst in conditions of industrial interest and to assess the feasibility of the process in a fluidized bed bioreactor (FBBR). The catalyst was first tested in a batch reactor at standard conditions and in various sets of conditions of interest for the process. Once the response of the catalyst to different operating conditions was tested and the operational stability assessed, one of the sets of conditions tested in batch was chosen for tests in FBBR. Prior to reaction tests, preliminary fluidization tests were realized in order to define an operating range of admissible flow rates. As a result, the FBR was run at different feed flow rates in a closed cycle configuration and its performance was compared to that of the batch system. The FBBR proved to be performing and suitable for scale up to large fructose production.
Keywords: enzyme reaction; inulin hydrolysis; fluidized bed reactor; fructose enzyme reaction; inulin hydrolysis; fluidized bed reactor; fructose
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Ricca, E.; Calabrò, V.; Curcio, S.; Basso, A.; Gardossi, L.; Iorio, G. Fructose Production by Inulinase Covalently Immobilized on Sepabeads in Batch and Fluidized Bed Bioreactor. Int. J. Mol. Sci. 2010, 11, 1180-1189.

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