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Int. J. Mol. Sci. 2010, 11(2), 647-655; doi:10.3390/ijms11020647
Article

Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall

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Received: 22 January 2010 / Accepted: 29 January 2010 / Published: 5 February 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Drywall from China has been reported to release sulfur producing products which are corrosive to metals, result in noxious odors, and represent a significant health risk. It has been reported that these emissions produce medical symptoms such as respiratory or asthma type problems, sinusitis, gastrointestinal disorders, and vision problems in home owners and their household pets. We report here a method of identifying a causative agent for these emissions by sampling affected gypsum wallboard and subjecting those samples to Real Time Polymerase Chain Reaction [RT-PCR] studies. Specific DNA probes and primers have been designed and patented that detect a specific iron and sulfur reducing bacterium (i.e., Thiobacillus ferrooxidans). One hundred percent of affected drywall samples obtained from homes located in the southeastern United States tested positive for the presence of T. ferrooxidans. All negative controls consisting of unaffected wallboard and internal controls, Geotrichum sp., tested negative within our limits of detection.
Keywords: Chinese drywall; Thiobacillus ferrooxidans; sulfur reducing bacteria; sulfur emissions Chinese drywall; Thiobacillus ferrooxidans; sulfur reducing bacteria; sulfur emissions
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hooper, D.G.; Shane, J.; Straus, D.C.; Kilburn, K.H.; Bolton, V.; Sutton, J.S.; Guilford, F.T. Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall. Int. J. Mol. Sci. 2010, 11, 647-655.

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