Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes

doi:10.3390/ijms11020427

This article is

freely available
re-usable

Int. J. Mol. Sci. 2010, 11(2), 427-457; doi:10.3390/ijms11020427

Review

Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes

Radek Macháň * and Martin Hof

J. Heyrovský Institute of Physical Chemistry of ASCR, v.v.i., Dolejškova 2155/3, 182 23 Prague, Czech Republic

^* Author to whom correspondence should be addressed.

Received: 3 December 2009; in revised form: 11 January 2010 / Accepted: 15 January 2010 / Published: 28 January 2010

(This article belongs to the Special Issue Single Molecules)

Download PDF Full-Text [712 KB, uploaded 28 January 2010 14:35 CET]

Abstract: Fluorescence correlation spectroscopy (FCS) is a single molecule technique used mainly for determination of mobility and local concentration of molecules. This review describes the specific problems of FCS in planar systems and reviews the state of the art experimental approaches such as 2-focus, Z-scan or scanning FCS, which overcome most of the artefacts and limitations of standard FCS. We focus on diffusion measurements of lipids and proteins in planar lipid membranes and review the contributions of FCS to elucidating membrane dynamics and the factors influencing it, such as membrane composition, ionic strength, presence of membrane proteins or frictional coupling with solid support.

Keywords: lateral diffusion; fluorescence fluctuation spectroscopy; confocal microscopy; biomembranes; supported lipid bilayers; giant unilamellar vesicles

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Macháň, R.; Hof, M. Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes. Int. J. Mol. Sci. 2010, 11, 427-457.

AMA Style

Macháň R., Hof M. Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes. International Journal of Molecular Sciences. 2010; 11(2):427-457.

Chicago/Turabian Style

Macháň, Radek; Hof, Martin. 2010. "Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes." Int. J. Mol. Sci. 11, no. 2: 427-457.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI Publishing, Basel, Switzerland RSS E-Mail Table of Contents Alert