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Int. J. Mol. Sci. 2010, 11(2), 386-406; doi:10.3390/ijms11020386
Article

Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy

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Received: 23 November 2009; in revised form: 17 January 2010 / Accepted: 21 January 2010 / Published: 28 January 2010
(This article belongs to the Special Issue Single Molecules)
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Abstract: Electrostatic interactions between dielectric surfaces and different fluorophores used in ultrasensitive fluorescence microscopy are investigated using objective-based Total Internal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacial dynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein, zwitterionic rhodamine 110 and neutral ATTO 488 are monitored at various ionic strengths at physiological pH. As analyzed by means of the amplitude and time-evolution of the autocorrelation function, the fluorescent molecules experience electrostatic attraction or repulsion at the glass surface depending on their charges. Influences of the electrostatic interactions are also monitored through the triplet-state population and triplet relaxation time, including the amount of detected fluorescence or the count-rate-per-molecule parameter. These TIR-FCS results provide an increased understanding of how fluorophores are influenced by the microenvironment of a glass surface, and show a promising approach for characterizing electrostatic interactions at interfaces.
Keywords: electrostatic interactions; fluorescent molecules; glass interfaces; Total Internal Reflection; Fluorescence Correlation Spectroscopy electrostatic interactions; fluorescent molecules; glass interfaces; Total Internal Reflection; Fluorescence Correlation Spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Blom, H.; Hassler, K.; Chmyrov, A.; Widengren, J. Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy. Int. J. Mol. Sci. 2010, 11, 386-406.

AMA Style

Blom H, Hassler K, Chmyrov A, Widengren J. Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy. International Journal of Molecular Sciences. 2010; 11(2):386-406.

Chicago/Turabian Style

Blom, Hans; Hassler, Kai; Chmyrov, Andriy; Widengren, Jerker. 2010. "Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy." Int. J. Mol. Sci. 11, no. 2: 386-406.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert