Next Article in Journal
The Fibrillar Collagen Family
Next Article in Special Issue
Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes
Previous Article in Journal
Phylogenetics Applied to Genotype/Phenotype Association and Selection Analyses with Sequence Data from Angptl4 in Humans
Previous Article in Special Issue
Single Molecule Experiments Challenge the Strict Wave-Particle Dualism of Light
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2010, 11(2), 386-406; doi:10.3390/ijms11020386

Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy

Department of Biomolecular Physics, Royal Institute of Technology, Stockholm, Sweden
Current address: SCANCO Medical AG, Fabrikweg 2, 8306 Brüttisellen, Switzerland
*
Authors to whom correspondence should be addressed.
Received: 23 November 2009 / Revised: 17 January 2010 / Accepted: 21 January 2010 / Published: 28 January 2010
(This article belongs to the Special Issue Single Molecules)
View Full-Text   |   Download PDF [355 KB, uploaded 19 June 2014]   |  

Abstract

Electrostatic interactions between dielectric surfaces and different fluorophores used in ultrasensitive fluorescence microscopy are investigated using objective-based Total Internal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacial dynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein, zwitterionic rhodamine 110 and neutral ATTO 488 are monitored at various ionic strengths at physiological pH. As analyzed by means of the amplitude and time-evolution of the autocorrelation function, the fluorescent molecules experience electrostatic attraction or repulsion at the glass surface depending on their charges. Influences of the electrostatic interactions are also monitored through the triplet-state population and triplet relaxation time, including the amount of detected fluorescence or the count-rate-per-molecule parameter. These TIR-FCS results provide an increased understanding of how fluorophores are influenced by the microenvironment of a glass surface, and show a promising approach for characterizing electrostatic interactions at interfaces.
Keywords: electrostatic interactions; fluorescent molecules; glass interfaces; Total Internal Reflection; Fluorescence Correlation Spectroscopy electrostatic interactions; fluorescent molecules; glass interfaces; Total Internal Reflection; Fluorescence Correlation Spectroscopy
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Blom, H.; Hassler, K.; Chmyrov, A.; Widengren, J. Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy. Int. J. Mol. Sci. 2010, 11, 386-406.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top