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Anti-Tumor Activity of a Novel Protein Obtained from Tartary Buckwheat
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi-214122, Jiangsu, China
School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 12 November 2010; in revised form: 18 November 2010 / Accepted: 14 December 2010 / Published: 17 December 2010
Abstract: TBWSP31 is a novel antitumor protein that was isolated from tartary buckwheat water-soluble extracts. The objective of this paper was to investigate the anti-proliferative effects of TBWSP31 on breast cancer Bcap37cells and to explore its possible mechanism. After treatment of Bcap37 cells with TBWSP31, typical apoptotic morphological changes were observed by inverted microscopy and scanning electron microscopy (SEM), such as detachment from the culture plate, change to a round shape, cell shrinkage, the absence of obvious microvilli, plasma membrane blebbing, and formation of apoptotic bodies. Cell-cycle analysis revealed that treatment with TBWSP31 resulted in a G0/G1 arrest and prevented the cells from growing from G0/G1 phase to S phase, which was most prominent at 48 h. The expression of bcl-2 and Fas were detected quantitatively by FCM, which showed that TBWSP31 induced-apoptosis may be involved with the participation of Fas and bcl-2. These results suggest that TBWSP31 is a potential antitumor compound and that apoptosis induced by TBWSP31 is a key antitumor mechanism.
Keywords: tartary buckwheat; protein; breast cancer cells; apoptosis; antitumor
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MDPI and ACS Style
Guo, X.; Zhu, K.; Zhang, H.; Yao, H. Anti-Tumor Activity of a Novel Protein Obtained from Tartary Buckwheat. Int. J. Mol. Sci. 2010, 11, 5201-5211.
Guo X, Zhu K, Zhang H, Yao H. Anti-Tumor Activity of a Novel Protein Obtained from Tartary Buckwheat. International Journal of Molecular Sciences. 2010; 11(12):5201-5211.
Guo, Xiaona; Zhu, Kexue; Zhang, Hui; Yao, Huiyuan. 2010. "Anti-Tumor Activity of a Novel Protein Obtained from Tartary Buckwheat." Int. J. Mol. Sci. 11, no. 12: 5201-5211.