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Int. J. Mol. Sci. 2010, 11(12), 4962-4972; doi:10.3390/ijms11124962

Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect

1
Biotechnology Department, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
2
Department of Breast Cancer Pathology and Research Laboratory, State Key Laboratory of Breast Cancer Research, Cancer Hospital of Tianjin Medical University, Tianjin 300060, China
3
Department of Medical and Bioengineering Science, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
*
Author to whom correspondence should be addressed.
Received: 21 October 2010 / Revised: 15 November 2010 / Accepted: 1 December 2010 / Published: 3 December 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation. View Full-Text
Keywords: sepiolite; carbon nanotube; plasmid transformation; nanomaterials; Yoshida effect sepiolite; carbon nanotube; plasmid transformation; nanomaterials; Yoshida effect
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Tan, H.; Fu, L.; Seno, M. Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect. Int. J. Mol. Sci. 2010, 11, 4962-4972.

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