Int. J. Mol. Sci. 2010, 11(12), 4962-4972; doi:10.3390/ijms11124962
Article

Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect

1email, 2email and 3,* email
Received: 21 October 2010; in revised form: 15 November 2010 / Accepted: 1 December 2010 / Published: 3 December 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.
Keywords: sepiolite; carbon nanotube; plasmid transformation; nanomaterials; Yoshida effect
PDF Full-text Download PDF Full-Text [240 KB, uploaded 19 June 2014 03:22 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Tan, H.; Fu, L.; Seno, M. Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect. Int. J. Mol. Sci. 2010, 11, 4962-4972.

AMA Style

Tan H, Fu L, Seno M. Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect. International Journal of Molecular Sciences. 2010; 11(12):4962-4972.

Chicago/Turabian Style

Tan, Haidong; Fu, Li; Seno, Masaharu. 2010. "Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect." Int. J. Mol. Sci. 11, no. 12: 4962-4972.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert