Myricetin Protects Cells against Oxidative Stress-Induced Apoptosis via Regulation of PI3K/Akt and MAPK Signaling Pathways
Abstract
:1. Introduction
2. Results and Discussion
2.1. Myricetin Inhibits H2O2-Induced Cell Death
2.2. Protective Effect of Myricetin against Apoptosis Induced by H2O2
2.3. Involvement of PI3K/Akt and MAPKs in the Anti-apoptotic Effect of Myricetin from H2O2 Treatment
3. Conclusions
4. Experimental Section
4.1. Reagents
4.2. Cell Culture
4.3. Cell Viability
4.4. Nuclear Staining with Hoechst 33342
4.5. DNA Fragmentation
4.6. Flow Cytometry Analysis
4.7. Mitochondrial Membrane Potential (ΔΨ) Analysis
4.8. Western Blot
4.9. Statistical Analysis
Acknowledgments
References
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Kang, K.A.; Wang, Z.H.; Zhang, R.; Piao, M.J.; Kim, K.C.; Kang, S.S.; Kim, Y.W.; Lee, J.; Park, D.; Hyun, J.W. Myricetin Protects Cells against Oxidative Stress-Induced Apoptosis via Regulation of PI3K/Akt and MAPK Signaling Pathways. Int. J. Mol. Sci. 2010, 11, 4348-4360. https://doi.org/10.3390/ijms11114348
Kang KA, Wang ZH, Zhang R, Piao MJ, Kim KC, Kang SS, Kim YW, Lee J, Park D, Hyun JW. Myricetin Protects Cells against Oxidative Stress-Induced Apoptosis via Regulation of PI3K/Akt and MAPK Signaling Pathways. International Journal of Molecular Sciences. 2010; 11(11):4348-4360. https://doi.org/10.3390/ijms11114348
Chicago/Turabian StyleKang, Kyoung Ah, Zhi Hong Wang, Rui Zhang, Mei Jing Piao, Ki Cheon Kim, Sam Sik Kang, Young Woo Kim, Jongsung Lee, Deokhoon Park, and Jin Won Hyun. 2010. "Myricetin Protects Cells against Oxidative Stress-Induced Apoptosis via Regulation of PI3K/Akt and MAPK Signaling Pathways" International Journal of Molecular Sciences 11, no. 11: 4348-4360. https://doi.org/10.3390/ijms11114348