Next Article in Journal
Distribution of Endogenous Farnesyl Pyrophosphate and Four Species of Lysophosphatidic Acid in Rodent Brain
Next Article in Special Issue
Lysophosphatidic Acid Level and the Incidence of Silent Brain Infarction in Patients with Nonvalvular Atrial Fibrillation
Previous Article in Journal
Pharmacological Proprieties of the Ethanol Extract of Muehlenbeckia platyclada (F. Muell.) Meisn. Leaves
Previous Article in Special Issue
Vaccenic and Elaidic Acid Modify Plasma and Splenocyte Membrane Phospholipids and Mitogen-Stimulated Cytokine Production in Obese Insulin Resistant JCR: LA-cp Rats
Int. J. Mol. Sci. 2010, 11(10), 3954-3964; doi:10.3390/ijms11103954
Article

The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet

1,* , 2
,
1
,
3
,
4
,
5
,
6
 and
7
1 School of Health Sciences, University of Wollongong, NSW 2522, Australia 2 Garvan Institute of Medical Research, Sydney, NSW 2010, Australia 3 School of Biological Sciences, University of Wollongong, NSW 2522, Australia 4 School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia 5 Department of Physical Education, Daegu University, Daegu, South Korea 6 Baker IDI Heart & Diabetes Institute, Melbourne, Victoria 3004 Australia 7 School of Chemistry, University of Wollongong, NSW 2522, Australia
* Author to whom correspondence should be addressed.
Received: 7 September 2010 / Revised: 8 October 2010 / Accepted: 12 October 2010 / Published: 15 October 2010
(This article belongs to the Special Issue Phospholipids: Molecular Sciences)
View Full-Text   |   Download PDF [318 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
Keywords: electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Mitchell, T.W.; Turner, N.; Else, P.L.; Hulbert, A.J.; Hawley, J.A.; Lee, J.S.; Bruce, C.R.; Blanksby, S.J. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. Int. J. Mol. Sci. 2010, 11, 3954-3964.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert