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The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet
School of Health Sciences, University of Wollongong, NSW 2522, Australia
Garvan Institute of Medical Research, Sydney, NSW 2010, Australia
School of Biological Sciences, University of Wollongong, NSW 2522, Australia
School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
Department of Physical Education, Daegu University, Daegu, South Korea
Baker IDI Heart & Diabetes Institute, Melbourne, Victoria 3004 Australia
School of Chemistry, University of Wollongong, NSW 2522, Australia
* Author to whom correspondence should be addressed.
Received: 7 September 2010; in revised form: 8 October 2010 / Accepted: 12 October 2010 / Published: 15 October 2010
Abstract: The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
Keywords: electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics
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Mitchell, T.W.; Turner, N.; Else, P.L.; Hulbert, A.J.; Hawley, J.A.; Lee, J.S.; Bruce, C.R.; Blanksby, S.J. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. Int. J. Mol. Sci. 2010, 11, 3954-3964.
Mitchell TW, Turner N, Else PL, Hulbert AJ, Hawley JA, Lee JS, Bruce CR, Blanksby SJ. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. International Journal of Molecular Sciences. 2010; 11(10):3954-3964.
Mitchell, Todd W.; Turner, Nigel; Else, Paul L.; Hulbert, Anthony J.; Hawley, John A.; Lee, Jong Sam; Bruce, Clinton R.; Blanksby, Stephen J. 2010. "The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet." Int. J. Mol. Sci. 11, no. 10: 3954-3964.