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Int. J. Mol. Sci. 2010, 11(10), 3954-3964; doi:10.3390/ijms11103954

The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet

1
School of Health Sciences, University of Wollongong, NSW 2522, Australia
2
Garvan Institute of Medical Research, Sydney, NSW 2010, Australia
3
School of Biological Sciences, University of Wollongong, NSW 2522, Australia
4
School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
5
Department of Physical Education, Daegu University, Daegu, South Korea
6
Baker IDI Heart & Diabetes Institute, Melbourne, Victoria 3004 Australia
7
School of Chemistry, University of Wollongong, NSW 2522, Australia
*
Author to whom correspondence should be addressed.
Received: 7 September 2010 / Revised: 8 October 2010 / Accepted: 12 October 2010 / Published: 15 October 2010
(This article belongs to the Special Issue Phospholipids: Molecular Sciences)
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Abstract

The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
Keywords: electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Mitchell, T.W.; Turner, N.; Else, P.L.; Hulbert, A.J.; Hawley, J.A.; Lee, J.S.; Bruce, C.R.; Blanksby, S.J. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. Int. J. Mol. Sci. 2010, 11, 3954-3964.

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