Abstract: The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
Keywords: electrospray-ionization mass spectrometry; endurance; exercise training; fatty acids; lipidomics
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Mitchell, T.W.; Turner, N.; Else, P.L.; Hulbert, A.J.; Hawley, J.A.; Lee, J.S.; Bruce, C.R.; Blanksby, S.J. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. Int. J. Mol. Sci. 2010, 11, 3954-3964.
Mitchell TW, Turner N, Else PL, Hulbert AJ, Hawley JA, Lee JS, Bruce CR, Blanksby SJ. The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. International Journal of Molecular Sciences. 2010; 11(10):3954-3964.
Mitchell, Todd W.; Turner, Nigel; Else, Paul L.; Hulbert, Anthony J.; Hawley, John A.; Lee, Jong Sam; Bruce, Clinton R.; Blanksby, Stephen J. 2010. "The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet." Int. J. Mol. Sci. 11, no. 10: 3954-3964.