Variations in IC50 Values with Purity of Mushroom Tyrosinase
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Materials
3.2. Methods
3.2.1. Crude Extract Tyrosinase Preparation
3.2.2. Enzyme Purification
3.2.3. Enzyme Assays
3.2.4. IC50 Determinations
4. Conclusions
Acknowledgments
Abbreviations:
ATTM | ammonium tetrathiomolybdate |
biphenol | 4,4’-dihydroxy-biphenyl |
DOPA | dihydroxyphenylalanine |
DMSO | dimethyl sulfoxide |
EDTA | ethylenediaminetetraacetic acid |
4HR | 4-hexylresorcinol |
MT | mushroom tyrosinase |
PMSF | phenylmethylsulfonyl fluoride |
SDS | sodium dodecylsulfate |
SHAM | salicyl-hydroxamic acid |
References and Notes
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Inhibitor | Crude MT μM | Commer. MT μM | Purified MT μM | Reported IC50 Values μM |
---|---|---|---|---|
kojic acid | 36 | 47 | 45 | 10–300 [10,18] |
SHAM | 0.24 | 0.4 | 0.45 | 0.45 [19], 0.8, 1.2 [10] |
4-HR | 5.8 | 1.5 | 1.2 | 0.85 [20], 2.5, 18 [10] |
tropolone | 0.39 | 1.2 | 0.95 | 0.4 [21], 1.3, 1.7 [10] |
methimazole | 46 | 55 | 50 | 200 [22], 40, 47 [10] |
ATTM | 3.4 | 3.3 | 2 | 2 [23], 7.5, 28 [10] |
NaCl | 65 mM | 270 mM | 360 mM | 25 mM [24] |
esculetin* | 70 | 280 | 685 | 43 [25], 210, 225 [10] |
biphenol* | 50a | 10b | 67c | 1.9 (tyrosine) [26] |
phloridzin* | 625 | 1740 | 1400 | 110 [27] |
hydroquinone | activatesd | activatese | activatesf | 26 [18] |
lysozyme | >10 mg/mLg | >20 mg/mLh | >20 mg/mLi | 0.32 μM [28] |
ZnSO4 | none@1 mM | none@1 mM | none@1 mM | 50 [29] |
anisaldehyde* | 1 mM | 1.4 mM | 1.8 mMj | 160 [30] |
DMSO | 2 M | 1.75 M | 1.4 M | 2.45 M [31] |
dimethyl sulfide | NI to 500 μM | NI to 500 μM | NI to 500 μM | |
dimethyl sulfone | NI to 2 mM | NI to 2 mM | NI to 2 mM | |
ethanol | 2.3 M | 2.1 M | 1.8 M |
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Neeley, E.; Fritch, G.; Fuller, A.; Wolfe, J.; Wright, J.; Flurkey, W. Variations in IC50 Values with Purity of Mushroom Tyrosinase. Int. J. Mol. Sci. 2009, 10, 3811-3823. https://doi.org/10.3390/ijms10093811
Neeley E, Fritch G, Fuller A, Wolfe J, Wright J, Flurkey W. Variations in IC50 Values with Purity of Mushroom Tyrosinase. International Journal of Molecular Sciences. 2009; 10(9):3811-3823. https://doi.org/10.3390/ijms10093811
Chicago/Turabian StyleNeeley, Elizabeth, George Fritch, Autumn Fuller, Jordan Wolfe, Jessica Wright, and William Flurkey. 2009. "Variations in IC50 Values with Purity of Mushroom Tyrosinase" International Journal of Molecular Sciences 10, no. 9: 3811-3823. https://doi.org/10.3390/ijms10093811