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Variations in IC50 Values with Purity of Mushroom Tyrosinase
Department of Chemistry and Physics, Indiana State University, Terre Haute, IN 47809, USA
* Author to whom correspondence should be addressed.
Received: 5 August 2009; in revised form: 19 August 2009 / Accepted: 31 August 2009 / Published: 2 September 2009
Abstract: The effects of various inhibitors on crude, commercial and partially purified commercial mushroom tyrosinase were examined by comparing IC50 values. Kojic acid, salicylhydroxamic acid, tropolone, methimazole, and ammonium tetrathiomolybdate had relatively similar IC50 values for the crude, commercial and partially purified enzyme. 4-Hexylresorcinol seemed to have a somewhat higher IC50 value using crude extracts, compared to commercial or purified tyrosinase. Some inhibitors (NaCl, esculetin, biphenol, phloridzin) showed variations in IC50 values between the enzyme samples. In contrast, hydroquinone, lysozyme, Zn2+, and anisaldehyde showed little or no inhibition in concentration ranges reported to be effective inhibitors. Organic solvents (DMSO and ethanol) had IC50 values that were similar for some of the tyrosinase samples. Depending of the source of tyrosinase and choice of inhibitor, variations in IC50 values were observed.
Keywords: mushroom; tyrosinase; inhibitors; IC50 values
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Neeley, E.; Fritch, G.; Fuller, A.; Wolfe, J.; Wright, J.; Flurkey, W. Variations in IC50 Values with Purity of Mushroom Tyrosinase. Int. J. Mol. Sci. 2009, 10, 3811-3823.
Neeley E, Fritch G, Fuller A, Wolfe J, Wright J, Flurkey W. Variations in IC50 Values with Purity of Mushroom Tyrosinase. International Journal of Molecular Sciences. 2009; 10(9):3811-3823.
Neeley, Elizabeth; Fritch, George; Fuller, Autumn; Wolfe, Jordan; Wright, Jessica; Flurkey, William. 2009. "Variations in IC50 Values with Purity of Mushroom Tyrosinase." Int. J. Mol. Sci. 10, no. 9: 3811-3823.