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Int. J. Mol. Sci. 2009, 10(7), 2896-2910; doi:10.3390/ijms10072896

Co-localization of Carbonic Anhydrase and Phosphoenol-pyruvate Carboxylase and Localization of Pyruvate Kinase in Roots and Hypocotyls of Etiolated Glycine max Seedlings

1
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece
2
Institute of Biology, Splaiul Independentei 296, Bucharest 060031, Romania
*
Author to whom correspondence should be addressed.
Received: 12 May 2009 / Revised: 17 June 2009 / Accepted: 26 June 2009 / Published: 29 June 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

We investigated the presence of carbonic anhydrase in root and hypocotyl of etiolated soybean using enzymatic, histochemical, immunohistochemical and in situ hybridization approaches. In parallel, we used in situ hybridization and immunolocaliza-tion to determine the expression pattern and localization of phosphoenolpyruvate carboxylase. Their co-localization in the root tip as well as in the central cylinder, suggests that a large fraction of the CO2 may be re-introduced into C4 compounds. GmPK3 expression, coding for a cytoplasmic isoform of pyruvate kinase, was detected in all different root cell types, suggesting that both phosphoenolpyruvate-utilizing enzymes are involved in phosphoenolpyruvate metabolism in etiolated soybean roots; a case indicative of the necessary flexibility plant metabolism has to adopt in order to compensate various physiological conditions.
Keywords: carbonic anhydrase; Glycine max; hypocotyl; in situ hybridization; phosphoenolpyruvate carboxylase; pyruvate kinase; root carbonic anhydrase; Glycine max; hypocotyl; in situ hybridization; phosphoenolpyruvate carboxylase; pyruvate kinase; root
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Dimou, M.; Paunescu, A.; Aivalakis, G.; Flemetakis, E.; Katinakis, P. Co-localization of Carbonic Anhydrase and Phosphoenol-pyruvate Carboxylase and Localization of Pyruvate Kinase in Roots and Hypocotyls of Etiolated Glycine max Seedlings. Int. J. Mol. Sci. 2009, 10, 2896-2910.

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