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Int. J. Mol. Sci. 2009, 10(6), 2752-2762; doi:10.3390/ijms10062752
Article

Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data

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Received: 19 May 2009 / Revised: 1 June 2009 / Accepted: 15 June 2009 / Published: 18 June 2009
(This article belongs to the Special Issue Isothermal Titration Calorimetry)
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Abstract

Current Isothermal Titration Calorimetry (ITC) data in the literature have relatively high errors in the measured enthalpies of protein-ligand binding reactions. There is a need for universal validation standards for titration calorimeters. Several inorganic salt co-precipitation and buffer protonation reactions have been suggested as possible enthalpy standards. The performances of several commercial calorimeters, including the VP-ITC, ITC200, and Nano ITC-III, were validated using these suggested standard reactions.
Keywords: titration calorimetry; enthalpy; protein-ligand binding; thermodynamics; carbonic anhydrase titration calorimetry; enthalpy; protein-ligand binding; thermodynamics; carbonic anhydrase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Baranauskienė, L.; Petrikaitė, V.; Matulienė, J.; Matulis, D. Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data. Int. J. Mol. Sci. 2009, 10, 2752-2762.

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