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Int. J. Mol. Sci. 2009, 10(6), 2752-2762; doi:10.3390/ijms10062752
Article

Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data

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Laboratory of Biothermodynamics and Drug Design / Institute of Biotechnology, Graičiūno 8, Vilnius, LT-02241, Lithuania
* Author to whom correspondence should be addressed.
Received: 19 May 2009 / Revised: 1 June 2009 / Accepted: 15 June 2009 / Published: 18 June 2009
(This article belongs to the Special Issue Isothermal Titration Calorimetry)
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Abstract

Current Isothermal Titration Calorimetry (ITC) data in the literature have relatively high errors in the measured enthalpies of protein-ligand binding reactions. There is a need for universal validation standards for titration calorimeters. Several inorganic salt co-precipitation and buffer protonation reactions have been suggested as possible enthalpy standards. The performances of several commercial calorimeters, including the VP-ITC, ITC200, and Nano ITC-III, were validated using these suggested standard reactions.
Keywords: titration calorimetry; enthalpy; protein-ligand binding; thermodynamics; carbonic anhydrase titration calorimetry; enthalpy; protein-ligand binding; thermodynamics; carbonic anhydrase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Baranauskienė, L.; Petrikaitė, V.; Matulienė, J.; Matulis, D. Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data. Int. J. Mol. Sci. 2009, 10, 2752-2762.

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