Int. J. Mol. Sci. 2009, 10(6), 2752-2762; doi:10.3390/ijms10062752
Article

Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data

Lina Baranauskienėemail, Vilma Petrikaitėemail, Jurgita Matulienėemail and Daumantas Matulis* email
Laboratory of Biothermodynamics and Drug Design / Institute of Biotechnology, Graičiūno 8, Vilnius, LT-02241, Lithuania
* Author to whom correspondence should be addressed.
Received: 19 May 2009; in revised form: 1 June 2009 / Accepted: 15 June 2009 / Published: 18 June 2009
(This article belongs to the Special Issue Isothermal Titration Calorimetry)
PDF Full-text Download PDF Full-Text [210 KB, uploaded 18 June 2009 04:20 CEST]
Abstract: Current Isothermal Titration Calorimetry (ITC) data in the literature have relatively high errors in the measured enthalpies of protein-ligand binding reactions. There is a need for universal validation standards for titration calorimeters. Several inorganic salt co-precipitation and buffer protonation reactions have been suggested as possible enthalpy standards. The performances of several commercial calorimeters, including the VP-ITC, ITC200, and Nano ITC-III, were validated using these suggested standard reactions.
Keywords: titration calorimetry; enthalpy; protein-ligand binding; thermodynamics; carbonic anhydrase

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Baranauskienė, L.; Petrikaitė, V.; Matulienė, J.; Matulis, D. Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data. Int. J. Mol. Sci. 2009, 10, 2752-2762.

AMA Style

Baranauskienė L, Petrikaitė V, Matulienė J, Matulis D. Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data. International Journal of Molecular Sciences. 2009; 10(6):2752-2762.

Chicago/Turabian Style

Baranauskienė, Lina; Petrikaitė, Vilma; Matulienė, Jurgita; Matulis, Daumantas. 2009. "Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data." Int. J. Mol. Sci. 10, no. 6: 2752-2762.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert