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Int. J. Mol. Sci. 2009, 10(6), 2662-2680; doi:10.3390/ijms10062662
Article

Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII

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Received: 25 March 2009; in revised form: 30 May 2009 / Accepted: 3 June 2009 / Published: 10 June 2009
(This article belongs to the Special Issue Isothermal Titration Calorimetry)
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Abstract: The analysis of tight protein-ligand binding reactions by isothermal titration calorimetry (ITC) and thermal shift assay (TSA) is presented. The binding of radicicol to the N-terminal domain of human heat shock protein 90 (Hsp90aN) and the binding of ethoxzolamide to human carbonic anhydrase (hCAII) were too strong to be measured accurately by direct ITC titration and therefore were measured by displacement ITC and by observing the temperature-denaturation transitions of ligand-free and ligand-bound protein. Stabilization of both proteins by their ligands was profound, increasing the melting temperature by more than 10 ºC, depending on ligand concentration. Analysis of the melting temperature dependence on the protein and ligand concentrations yielded dissociation constants equal to 1 nM and 2 nM for Hsp90aN-radicicol and hCAII-ethoxzolamide, respectively. The ligand-free and ligand-bound protein fractions melt separately, and two melting transitions are observed. This phenomenon is especially pronounced when the ligand concentration is equal to about half the protein concentration. The analysis compares ITC and TSA data, accounts for two transitions and yields the ligand binding constant and the parameters of protein stability, including the Gibbs free energy and the enthalpy of unfolding.
Keywords: Hsp90; radicicol; thermal shift assay; isothermal titration calorimetry; protein-ligand binding; carbonic anhydrase; ethoxzolamide Hsp90; radicicol; thermal shift assay; isothermal titration calorimetry; protein-ligand binding; carbonic anhydrase; ethoxzolamide
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zubrienė, A.; Matulienė, J.; Baranauskienė, L.; Jachno, J.; Torresan, J.; Michailovienė, V.; Cimmperman, P.; Matulis, D. Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII. Int. J. Mol. Sci. 2009, 10, 2662-2680.

AMA Style

Zubrienė A, Matulienė J, Baranauskienė L, Jachno J, Torresan J, Michailovienė V, Cimmperman P, Matulis D. Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII. International Journal of Molecular Sciences. 2009; 10(6):2662-2680.

Chicago/Turabian Style

Zubrienė, Asta; Matulienė, Jurgita; Baranauskienė, Lina; Jachno, Jelena; Torresan, Jolanta; Michailovienė, Vilma; Cimmperman, Piotras; Matulis, Daumantas. 2009. "Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII." Int. J. Mol. Sci. 10, no. 6: 2662-2680.



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