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Purification, Crystallization and Preliminary X-ray Crystallographic Studies of RAIDD Death-Domain (DD)
Department of Biochemistry, School of Biotechnology at Yeungnam University, Gyeong-san, Korea
* Author to whom correspondence should be addressed.
Received: 7 April 2009; in revised form: 26 May 2009 / Accepted: 1 June 2009 / Published: 3 June 2009
Abstract: Caspase-2 activation by formation of PIDDosome is critical for genotoxic stress induced apoptosis. PIDDosome is composed of three proteins, RAIDD, PIDD, and Caspase-2. RAIDD is an adaptor protein containing an N-terminal Caspase-Recruiting-Domain (CARD) and a C-terminal Death-Domain (DD). Its interactions with Caspase-2 and PIDD through CARD and DD respectively and formation of PIDDosome are important for the activation of Caspase-2. RAIDD DD cloned into pET26b vector was expressed in E. coli cells and purified by nickel affinity chromatography and gel filtration. Although it has been known that the most DDs are not soluble in physiological condition, RAIDD DD was soluble and interacts tightly with PIDD DD in physiological condition. The purified RAIDD DD alone has been crystallized. Crystals are trigonal and belong to space group P3121 (or its enantiomorph P3221) with unit-cell parameters a = 56.3, b = 56.3, c = 64.9 Å and γ = 120°. The crystals were obtained at room temperature and diffracted to 2.0 Å resolution.
Keywords: RAIDD; PIDDosome; crystallization; Death-Domain (DD)
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MDPI and ACS Style
Jang, T.-H.; Park, H.H. Purification, Crystallization and Preliminary X-ray Crystallographic Studies of RAIDD Death-Domain (DD). Int. J. Mol. Sci. 2009, 10, 2501-2509.
Jang T-H, Park HH. Purification, Crystallization and Preliminary X-ray Crystallographic Studies of RAIDD Death-Domain (DD). International Journal of Molecular Sciences. 2009; 10(6):2501-2509.
Jang, Tae-ho; Park, Hyun Ho. 2009. "Purification, Crystallization and Preliminary X-ray Crystallographic Studies of RAIDD Death-Domain (DD)." Int. J. Mol. Sci. 10, no. 6: 2501-2509.